Plant Molecular Biology Laboratory, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India.
Biotechnol Lett. 2011 Aug;33(8):1689-97. doi: 10.1007/s10529-011-0620-x. Epub 2011 Apr 29.
Stress responsive transcriptional regulation is an adaptive strategy of plants that alleviates the adverse effects of environmental stresses. The ectopic overexpression of Dehydration-Responsive Element Binding transcription factors (DREBs) either in homologous or in heterologous plants improved stress tolerance indicating the DRE/DREB regulon is conserved across plants. We developed 30 transgenic T(0) rice plants overexpressing OsDREB2A which were devoid of any growth penalty or phenotypic abnormalities during stressed or non-stressed conditions. Integration of T-DNA in the rice genome and stress inducible overexpression of OsDREB2A had occurred in these transgenic lines. Functional analyses of T(1)-3 and T(1)-10 lines revealed significant tolerance to osmotic, salt and dehydration stresses during simulated stress conditions with enhanced growth performance as compared to wild type. OsDREB2A, thus, confers stress tolerance in homologous rice system that failed in the heterologous Arabidopsis system earlier.
胁迫响应转录调控是植物的一种适应性策略，可减轻环境胁迫的不利影响。在同源或异源植物中外源过表达脱水应答元件结合转录因子（DREB）可提高胁迫耐受性，表明 DRE/DREB 调控元件在植物中是保守的。我们开发了 30 个转 OsDREB2A 基因的 T(0) 水稻植株，这些植株在胁迫或非胁迫条件下没有任何生长缺陷或表型异常。在这些转基因系中，T-DNA 整合到水稻基因组中，并在胁迫下诱导 OsDREB2A 过表达。T(1)-3 和 T(1)-10 系的功能分析表明，与野生型相比，它们在模拟胁迫条件下对渗透、盐和干旱胁迫具有显著的耐受性，生长性能得到增强。因此，OsDREB2A 在同源水稻系统中赋予了胁迫耐受性，而在先前的异源拟南芥系统中却没有。
