Hagen T J, Shimkets L J
Department of Microbiology, University of Georgia, Athens 30602.
J Bacteriol. 1990 Jan;172(1):15-23. doi: 10.1128/jb.172.1.15-23.1990.
The csg locus of Myxococcus xanthus appears to control the production of an intercellular signal that is essential for development. The complete nucleotide sequence of a clone containing the csg locus was determined by the dideoxy-chain termination method. Pattern recognition analyses of the DNA sequence revealed the presence of two protein-coding regions that are convergently oriented and separated by only 8 nucleotides. Tn5 lac insertions into this clone detected two transcriptional units that are transcribed in a convergent fashion and whose expression increases during development. The two genes represented by these protein-coding regions and transcriptional units have been designated csgA and fprA. Northern (RNA) blot analyses detected an 800-nucleotide RNA specific to the csgA gene and a 900-nucleotide RNA specific to the fprA gene. Our results, along with mutational studies, identify csgA as the gene involved in cell communication. The function of the fprA gene is described in an accompanying paper (L. J. Shimkets, J. Bacteriol. 172:24-30, 1990).
黄色黏球菌的csg基因座似乎控制着一种对发育至关重要的细胞间信号的产生。通过双脱氧链终止法测定了包含csg基因座的一个克隆的完整核苷酸序列。对该DNA序列的模式识别分析揭示了存在两个蛋白质编码区，它们呈反向排列且仅相隔8个核苷酸。向该克隆中插入Tn5 lac检测到两个转录单元，它们以反向方式转录，并且其表达在发育过程中增加。由这些蛋白质编码区和转录单元所代表的两个基因已被命名为csgA和fprA。Northern（RNA）印迹分析检测到一种特异于csgA基因的800个核苷酸的RNA和一种特异于fprA基因的900个核苷酸的RNA。我们的结果以及突变研究确定csgA是参与细胞通讯的基因。fprA基因的功能在一篇配套论文中进行了描述（L. J. Shimkets，《细菌学杂志》172:24 - 30，1990）。
