Condit C, Hagen T J, McKnight T D, Meagher R B
Gene. 1983 Nov;25(1):101-8. doi: 10.1016/0378-1119(83)90172-5.
Using Northern blot analysis we have studied the transcription of the CM4-184 Ga. strain of cauliflower mosaic virus (CaMV) DNA. This analysis reveals that this CaMV strain, like the Cabb-BS and the Cabb B-JI strains, produces both a genomic length transcript and a 1900-nucleotide (nt) transcript during infection. In addition, we detect an 1800-nt PA+ transcript mapping primarily to the EcoRI-c region of the virus, and three apparent minor viral-specific PA + RNAs of 4900, 4500, and 4300 nt. We also report the presence of two small viral single-stranded DNAs produced during infection, and show that both DNAs are derived from the EcoRI-b region of the virus.
我们利用Northern印迹分析研究了花椰菜花叶病毒（CaMV）CM4 - 184 Ga株系DNA的转录情况。该分析表明，此CaMV株系与Cabb - BS和Cabb B - JI株系一样，在感染过程中会产生基因组长度的转录本和一个1900个核苷酸（nt）的转录本。此外，我们检测到一个主要定位于病毒EcoRI - c区域的1800 nt的PA +转录本，以及三个明显较小的病毒特异性PA + RNA，大小分别为4900 nt、4500 nt和4300 nt。我们还报告了感染过程中产生的两种小的病毒单链DNA的存在，并表明这两种DNA均源自病毒的EcoRI - b区域。
