Bhattacharyya M K, Smith A M, Ellis T H, Hedley C, Martin C
John Innes Institute, Norwich, United Kingdom.
Cell. 1990 Jan 12;60(1):115-22. doi: 10.1016/0092-8674(90)90721-p.
We describe the cloning of the r (rugosus) locus of pea (Pisum sativum L.), which determines whether the seed is round or wrinkled. Wrinkled (rr) seeds lack one isoform of starch-branching enzyme (SBEI), present in round (RR or Rr) seeds. A major polymorphism in the SBEI gene between near-isogenic RR and rr lines shows 100% cosegregation with the r locus, establishing that the SBEI gene is at the r locus. An aberrant transcript for SBEI is produced in rr embryos. In rr lines the SBEI gene is interrupted by a 0.8 kb insertion that is very similar to the Ac/Ds family of transposable elements from maize. Failure to produce SBEI has complex metabolic consequences on starch, lipid, and protein biosynthesis in the seed.
我们描述了豌豆（Pisum sativum L.）皱粒（r）基因座的克隆，该基因座决定种子是圆形还是皱缩形。皱粒（rr）种子缺乏淀粉分支酶（SBEI）的一种同工型，而这种同工型存在于圆粒（RR或Rr）种子中。近等基因RR系和rr系之间的SBEI基因存在一个主要多态性，它与r基因座完全共分离，这表明SBEI基因位于r基因座上。在rr胚胎中产生了一种异常的SBEI转录本。在rr品系中，SBEI基因被一个0.8 kb的插入片段打断，该插入片段与玉米的Ac/Ds转座元件家族非常相似。无法产生SBEI会对种子中的淀粉、脂质和蛋白质生物合成产生复杂的代谢影响。
