Department of Infectious Diseases and Clinical Microbiology, Zonguldak Karaelmas University, Turkey.
Am J Infect Control. 2011 Nov;39(9):757-62. doi: 10.1016/j.ajic.2010.12.003. Epub 2011 Apr 29.
The aim of this study was to evaluate the activity of a dry mist-generated hydrogen peroxide (DMHP) system (Sterinis; Gloster Sante Europe, Labege cedex, France) against methicillin-resistant Staphylococcus aureus (MRSA) and Acinetobacter baumannii.
McFarland 0.5 suspensions of 2 test bacteria, either pure or containing 5% sterile serum, were prepared and inoculated onto sterile stainless steel disks. Each disk in a Petri dish-with the Petri dish cover either closed or open-was placed in different locations in an intensive care unit room. Quantitative cultures were performed after the cycle.
No growth occurred on the disks in the absence of a barrier, except 1 disk containing serum. Existence of a barrier, as a drawer or a covered Petri dish, caused failure in the disinfection activity. The mean reduction in initial log(10) bacterial count was lower for both of the test bacteria in presence of a barrier: 4.44- to 4.70-log(10) colony-forming units (cfu) decrease was observed in absence of a barrier, whereas 1.49- to 3.79-log(10) cfu decrease was observed in presence of a barrier. When the culture results were compared according to organic load content, the mean (±standard deviation) reduction of initial contamination in pure and in serum containing MRSA suspensions was 4.25 ± 1.20- and 3.34 ± 1.89-log(10) cfu, respectively. The mean (±standard deviation) reduction in pure and in serum containing A baumannii suspensions was 4.34 ± 0.89- and 3.87 ± 1.26-log(10) cfu, respectively. The differences were statistically significant (P < .001).
Sterinis was capable of killing MRSA and A baumannii on open surfaces; however, it was not effective in closed or semiclosed areas. Presence of serum also caused failure in the disinfection activity of the system.
本研究旨在评估一种干燥雾化产生的过氧化氢（DMHP）系统（Sterinis；Gloster Sante Europe，Labege cedex，法国）对耐甲氧西林金黄色葡萄球菌（MRSA）和鲍曼不动杆菌的活性。
制备 2 种测试细菌的 McFarland 0.5 悬浮液，要么是纯悬浮液，要么是含有 5%无菌血清的悬浮液，然后将其接种到无菌不锈钢圆盘上。在培养皿中，每个圆盘（培养皿盖要么关闭，要么打开）放置在重症监护病房的不同位置。循环后进行定量培养。
在没有屏障的情况下，除了含有血清的 1 个圆盘外，圆盘上没有生长。存在屏障（如抽屉或覆盖的培养皿）会导致消毒活性失败。在存在屏障的情况下，两种测试细菌的初始对数（10）细菌计数减少平均值较低：在不存在屏障的情况下观察到 4.44-4.70 对数（10）菌落形成单位（cfu）的减少，而在存在屏障的情况下观察到 1.49-3.79 对数（10）cfu 的减少。根据有机负荷含量比较培养结果时，在纯 MRSA 悬浮液和含血清的 MRSA 悬浮液中，初始污染的平均（±标准偏差）减少量分别为 4.25 ± 1.20-和 3.34 ± 1.89-对数（10）cfu。在纯 A 鲍曼不动杆菌悬浮液和含血清的 A 鲍曼不动杆菌悬浮液中，初始污染的平均（±标准偏差）减少量分别为 4.34 ± 0.89-和 3.87 ± 1.26-对数（10）cfu。差异具有统计学意义（P <.001）。
Sterinis 能够杀死开放表面的 MRSA 和 A 鲍曼不动杆菌；然而，它在封闭或半封闭区域无效。血清的存在也会导致系统消毒活性失败。
