Department of Burn Surgery, Hangang Sacred Heart Hospital, Hallym University Medical Center, Youngdeungpo-gu, Seoul, Republic of Korea.
Burns. 2011 Sep;37(6):1067-71. doi: 10.1016/j.burns.2011.03.018. Epub 2011 Apr 29.
We address the clinical application of the suspension type cultured epithelial autografts (CEAs), Keraheal™ (MCTT, Seoul, Korea), along with the effects, application method, merits and demerits thereof. From February 2007 to June 2010, 29 burn patients with extensive burns, participated in the suspension type of CEA clinical test. A widely meshed autograft (1:4-6 ratio) was applied to the wound bed and the suspension type CEA was sprayed with a Tissomat cell sprayer, followed by a Tissucol spray, a fibrin sealant. The patients' (men/women=26/3) median (interquartile ranges) age was 42 (30-49) years old, the burned TBSA was 55 (44-60) %, and the full thickness burn area was 40 (30-46.5) %. The area of Keraheal™ applied was 800 (400-1200) cm(2). The take rate was 96 (90.5-99) % and 100 (98.5-100) % at 2 and 4 weeks after treatment with Keraheal™, respectively. The Vancouver burn scar scale was 5 (4-6.5), 4 (3-6), and 3 (2-4) at 8, 12 and 24 weeks after the Keraheal™ application. Widely meshed autograft must be applied in massive burns but it's take rate is greatly reduced. The CEAs enhance the take rate of a wide meshed autograft in massive burns and allow for grafting wide meshed autograft together with acellular dermal matrix in some cases.
我们探讨了悬浮培养上皮同种移植物（CEAs），即 Keraheal™（MCTT，韩国首尔）的临床应用，以及其效果、应用方法、优缺点。2007 年 2 月至 2010 年 6 月，29 例大面积烧伤患者参与了悬浮型 CEA 临床测试。将广泛网状自体移植物（1:4-6 比例）应用于创面床，并用 Tissomat 细胞喷雾器喷洒悬浮型 CEA，然后喷洒 Tissucol、纤维蛋白密封剂。患者（男/女=26/3）的中位（四分位间距）年龄为 42（30-49）岁，烧伤总面积 TBSA 为 55（44-60）%，全厚烧伤面积为 40（30-46.5）%。应用 Keraheal™的面积为 800（400-1200）cm²。Keraheal™治疗后 2 周和 4 周的成活率分别为 96（90.5-99）%和 100（98.5-100）%。Keraheal™应用后 8、12 和 24 周的温哥华烧伤瘢痕量表评分分别为 5（4-6.5）、4（3-6）和 3（2-4）。大面积烧伤必须应用广泛网状自体移植物，但成活率大大降低。CEAs 可提高大面积烧伤广泛网状自体移植物的成活率，并在某些情况下允许与脱细胞真皮基质一起进行广泛网状自体移植物移植。
