Camacho A, Spear G
Cell. 1978 Nov;15(3):993-1002. doi: 10.1016/0092-8674(78)90283-0.
Isolated restriction endonuclease fragments of the herpes simplex virus type 1 (HSV-1) genome were introduced into hamster embryo cells to identify DNA sequences capable to transforming the cells with respect to acquisition of properties correlated with tumorigenicity. One of the fragments generated by cleavage of HSV-1 DNA with the restriction endonuclease Xba I was found to induce transformation at a frequency of about 10 colonies per quantity of fragment recovered from 1 microgram of uncut DNA; fractions containing the other Xba I fragments failed to induce transformation reproducibly, although occasional colonies were detected. The fragment with transforming activity (Xba I-F) is 15.5 X 10(6) daltons in molecular weight and is located between 0.30 and 0.45 map units on the HSV-1 genome. The Xba I-F transformants obtained were selected for their ability to replicate in low concentrations of serum; in addition, they were found to attain high saturation densities in the presence of 10% serum and to form colonies in semisolid medium. Moreover, the transformed cells produced at least one of the viral gene products (a membrane glycoprotein) encoded in the fragment used for transformation, indicating not only that viral DNA was incorporated into the cells, but also that viral genes were expressed.
将单纯疱疹病毒1型（HSV-1）基因组的限制性内切酶片段分离出来，导入仓鼠胚胎细胞，以鉴定能够使细胞获得与致瘤性相关特性的DNA序列。用限制性内切酶Xba I切割HSV-1 DNA产生的片段之一，以每从1微克未切割DNA中回收的片段量约10个菌落的频率诱导转化；含有其他Xba I片段的组分未能可重复地诱导转化，尽管偶尔能检测到菌落。具有转化活性的片段（Xba I-F）分子量为15.5×10⁶道尔顿，位于HSV-1基因组的0.30至0.45个图谱单位之间。获得的Xba I-F转化体因其在低浓度血清中复制的能力而被筛选；此外，发现它们在10%血清存在下能达到高饱和密度，并在半固体培养基中形成菌落。此外，转化细胞产生了用于转化的片段中编码的至少一种病毒基因产物（一种膜糖蛋白），这不仅表明病毒DNA已整合到细胞中，而且表明病毒基因已表达。
