Chen Lin, Tang Xi-Xiang, Zheng Min, Yi Zhi-Wei, Xiao Xiang, Qiu Ying-Kun, Wu Zhen
Medical College, Xiamen University, China.
J Asian Nat Prod Res. 2011 May;13(5):444-8. doi: 10.1080/10286020.2011.570262.
To explore secondary metabolites in deep-sea sediment metagenomic clone-derived Escherichia coli fermentation broth, different kinds of chromatography methods were used in the isolation procedures, while the structures of the isolated compounds were assigned based on the MS analysis and their (1)H and (13)C NMR spectra including 2D NMR techniques such as COSY, HMQC, and HMBC experiments. As a result, a novel compound was isolated and characterized as N-{1-[4-(acetylamino)phenyl]-3-hydroxy-1-(1H-indol-3-yl)propan-2-yl}-2,2-dichloroacetamide (1). In addition, eight known compounds were also obtained. Fatty acid amide hydrolase and monoacylglycerol lipase were used to screen analgesic activity, and the new compound showed analgesic activity to some extent in pharmacological test.
为了探索深海沉积物宏基因组克隆来源的大肠杆菌发酵液中的次生代谢产物，在分离过程中使用了不同类型的色谱方法，同时基于质谱分析及其一维氢谱和碳谱（包括二维核磁共振技术，如COSY、HMQC和HMBC实验）对分离得到的化合物结构进行了鉴定。结果，分离得到了一种新型化合物，并将其鉴定为N-{1-[4-(乙酰氨基)苯基]-3-羟基-1-(1H-吲哚-3-基)丙-2-基}-2,2-二氯乙酰胺（1）。此外，还获得了8种已知化合物。使用脂肪酸酰胺水解酶和单酰甘油脂肪酶筛选镇痛活性，新化合物在药理试验中显示出一定程度的镇痛活性。
