Dept. of Pediatrics, Yuan's General Hospital, Taiwan.
J Food Sci. 2010 Oct;75(8):H254-9. doi: 10.1111/j.1750-3841.2010.01812.x.
There is increasing evidence that daily intake of flavonoids reduced severity and prevalence of allergic diseases. However, the mechanism of its antiinflammatory effects in allergic diseases remains uncertain. Kaempferol, which belongs to the flavone group, is a strong antioxidant among natural flavonoids and is the essential component of many beverages and vegetables. Because chemokine is one of the key mediators in allergic inflammatory process, we investigated the effect of kaempferol on chemokines expression in monocytes. Our data demonstrated that kaempferol significantly inhibited the lipopolysaccharide (LPS)-induced production of monocyte-derived chemokine (MDC), interferon gamma-induced protein 10 (IP-10), and interleukin-8 (IL-8) in THP-1 cells. Growth-related oncogene-α (GRO-α) was also suppressed at a higher concentration. We also found that kaempferol was able to suppress LPS-induced mitogen-activated protein kinase (MAPK) pathways, as well as the phosphorylation of upstream c-raf and MEK1/2. In brief, kaempferol suppressed LPS-induced T helper 1 (Th1), T helper 2 (Th2), and neutrophil-related chemokines production in monocytes might be via the MAPK pathways.
越来越多的证据表明，黄酮类化合物的日常摄入可以减轻过敏疾病的严重程度和患病率。然而，其在过敏疾病中的抗炎作用机制尚不确定。山柰酚属于黄酮类，是天然类黄酮中较强的抗氧化剂，也是许多饮料和蔬菜的重要成分。由于趋化因子是过敏炎症过程中的关键介质之一，我们研究了山柰酚对单核细胞趋化因子表达的影响。我们的数据表明，山柰酚可显著抑制脂多糖（LPS）诱导的单核细胞衍生趋化因子（MDC）、干扰素γ诱导蛋白 10（IP-10）和白细胞介素-8（IL-8）的产生。生长相关癌基因-α（GRO-α）在更高浓度时也受到抑制。我们还发现，山柰酚能够抑制 LPS 诱导的丝裂原活化蛋白激酶（MAPK）通路，以及上游 c-raf 和 MEK1/2 的磷酸化。总之，山柰酚可能通过 MAPK 通路抑制单核细胞中 LPS 诱导的 Th1、Th2 和嗜中性粒细胞相关趋化因子的产生。
