Department of Science, Wesley College, Dover, DE 19901, USA.
J Microbiol Methods. 2011 Aug;86(2):140-4. doi: 10.1016/j.mimet.2011.04.009. Epub 2011 Apr 20.
The measurement of elemental selenium (Se(0)) is needed to assess the rate and magnitude of bacteria reduction of selenite or selenate. We have developed a spectrophotometric method for the measurement Se(0) that is rapid and can be employed to measure the quantity of Se(0) produced by bacterial cultures. This method employs the use of 1M Na(2)S to convert the insoluble elemental selenium to a red-brown solution and with this method there is a direct correlation between concentration of elemental selenium and the absorption at 500nm. To demonstrate the utility of this assay, we have followed the reduction of selenite to Se(0) by Moraxella bovis, and by bacterial consortia in soil and water samples.
需要测量元素硒（Se(0)）来评估亚硒酸盐或硒酸盐被细菌还原的速率和程度。我们已经开发出一种快速的分光光度法来测量 Se(0)，可用于测量细菌培养物产生的 Se(0)量。该方法使用 1M Na(2)S 将不溶性元素硒转化为红棕色溶液，并且该方法中元素硒的浓度与 500nm 处的吸光度之间存在直接相关性。为了证明该测定法的实用性，我们已经通过莫拉氏菌和土壤和水样中的细菌群落来跟踪亚硒酸盐到 Se(0)的还原过程。
