Yang Jun, Ding Mei, Sun Yin, Pang Hao, Xing Jia-xin, Xuan Jin-feng, Li Chun-mei, Wang Bao-jie
School of Forensic Medicine, China Medical University, Shenyang 110001, China.
Fa Yi Xue Za Zhi. 2011 Feb;27(1):22-4.
To investigate the polymorphism of cholecystokinin (CCK) gene -45C/T of schizophrenia and its application in forensic medicine.
Bidirectional allele specific PCR was used to detect CCK gene -45C/T polymorphisms in 207 schizophrenic patients (case group) and 202 healthy individuals (control group) of the Han population in northern China. The chi2 test was used to identify Hardy-Weinberg equilibrium of the genotype distribution in control group. The differences of genotype and allele frequencies distributions were compared between two groups.
Distributions of the genotype frequencies satisfied the law of Hardy-Weinberg equilibrium in control group. The differences between genotypic frequencies and allele frequencies were not statistical significance in case group and control groups (P > 0.05). Gender-stratified analysis showed that frequency of allele T in female case group was statistically higher than that in female control group (P = 0.044).
CCK gene -45C/T locus T allele may be positively associated with schizophrenia in female population and useful in schizophrenia identification.
探讨精神分裂症患者胆囊收缩素（CCK）基因-45C/T多态性及其在法医学中的应用。
采用双向等位基因特异性PCR技术，检测中国北方汉族人群207例精神分裂症患者（病例组）和202例健康个体（对照组）的CCK基因-45C/T多态性。采用卡方检验分析对照组基因型分布是否符合Hardy-Weinberg平衡。比较两组基因型和等位基因频率分布的差异。
对照组基因型频率分布符合Hardy-Weinberg平衡定律。病例组与对照组的基因型频率和等位基因频率差异无统计学意义（P>0.05）。按性别分层分析显示，女性病例组等位基因T的频率显著高于女性对照组（P=0.044）。
CCK基因-45C/T位点的T等位基因可能与女性精神分裂症呈正相关，对精神分裂症的个体识别有一定意义。
