Dahamna S, Harzallah D, Boussahel S, Belgeit A, Merghem M, Bouriche H
Department of Biology, Faculty of Sciences, University Ferhat Abbas, Sétif 19000, Algeria.
Commun Agric Appl Biol Sci. 2010;75(2):203-7.
Cypermetherin is widely used in Algeria; this insecticide belongs to the group of pyrethroids classified by the World Health Organization as moderately harmful class II (WHO, 2005). This study was conducted to search the effect of non lethal dose on biochemical parameters, hematological and histological parts of the organs. Male of domestic rabbits Oryctolagus cuniculus (1 kg) received per week and per gavage 1/10 LD50 of Cypermetherin (ARRIVO 25%, active substance of Cypermetherin 250 g/l). blood was collected 72 hours after the treatment. Enzyme activities were assayed in the plasma samples for Glutamate oxalo acetate transaminase (GOT), Glutamate pyruvate transaminase (GPT), Alcaline phosphatase (AlcP), creatinine (Crea) and glucose. Red blood (RBC) cells and white blood cells (WBC) were calculated too. The results indicated a significant increase in transaminases GOT and GPT, and AlcP explain a high energy generating product and dysfunction of the liver. A decline in Crea, Hb, RBC and WBC which is related to the immunity, this is probably due to cell lyses explain the effect of Cypermetherin on erythropoeisis. Histological examination confirmed the biochemical tests by the observation of inflammatory infiltrate and perilobular fibrosis. In conclusion, Cypermetherin with used dose affects biochemical, hematological and histological parameters of the rabbits.
氯氰菊酯在阿尔及利亚被广泛使用；这种杀虫剂属于拟除虫菊酯类，世界卫生组织将其列为中等危害的II类（世界卫生组织，2005年）。本研究旨在探究非致死剂量对家兔生化参数、血液学和器官组织学方面的影响。雄性家兔（穴兔属，体重1千克）每周经口灌胃给予1/10 LD50的氯氰菊酯（ARRIVO 25%，氯氰菊酯活性成分250克/升）。在处理后72小时采集血液。检测血浆样本中的谷氨酸草酰乙酸转氨酶（GOT）、谷氨酸丙酮酸转氨酶（GPT）、碱性磷酸酶（AlcP）、肌酐（Crea）和葡萄糖的酶活性。同时计算红细胞（RBC）和白细胞（WBC）数量。结果表明，转氨酶GOT和GPT以及AlcP显著升高，这解释了高能量生成产物以及肝脏功能障碍。Crea、血红蛋白（Hb）、RBC和WBC下降，这与免疫力相关，这可能是由于细胞溶解解释了氯氰菊酯对红细胞生成的影响。组织学检查通过观察炎症浸润和小叶周围纤维化证实了生化检测结果。总之，所用剂量的氯氰菊酯会影响家兔的生化、血液学和组织学参数。
