声悬浮微晶体可获得高分辨率的 X 射线结构。
Acoustically mounted microcrystals yield high-resolution X-ray structures.
机构信息
Biology Department, Brookhaven National Laboratory, Upton, NY 11973-5000, USA.
出版信息
Biochemistry. 2011 May 31;50(21):4399-401. doi: 10.1021/bi200549x. Epub 2011 May 9.
Abstract
We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micrometer X-ray beam across the cryocooled micromeshes. X-ray diffraction data sets merged from several micrometer-sized crystals are used to determine 1.8 Ǻ resolution crystal structures.
摘要
我们展示了一种从微晶体雨水中确定结构的通用策略。它使用声滴喷射将 2.5nL 的液滴滴从微晶体悬浮液的表面转移到安装的微网针上，穿过空气。通过在低温微网上扫描几微米的 X 射线光束来定位单个微晶体。来自几个微米大小的晶体的 X 射线衍射数据集被合并以确定 1.8Å分辨率的晶体结构。
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