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使用声学液滴喷射减少串行晶体学的样品消耗。

Reducing sample consumption for serial crystallography using acoustic drop ejection.

作者信息

Davy Bradley, Axford Danny, Beale John H, Butryn Agata, Docker Peter, Ebrahim Ali, Leen Gabriel, Orville Allen M, Owen Robin L, Aller Pierre

机构信息

Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE, UK.

PolyPico Technologies Ltd, Unit 10, Airways Technology Park, Rathmacullig Wes, Ballygarvan, Cork T12 DY95, Ireland.

出版信息

J Synchrotron Radiat. 2019 Sep 1;26(Pt 5):1820-1825. doi: 10.1107/S1600577519009329. Epub 2019 Aug 16.

DOI:10.1107/S1600577519009329
PMID:31490175
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6730619/
Abstract

Efficient sample delivery is an essential aspect of serial crystallography at both synchrotrons and X-ray free-electron lasers. Rastering fixed target chips through the X-ray beam is an efficient method for serial delivery from the perspectives of both sample consumption and beam time usage. Here, an approach for loading fixed targets using acoustic drop ejection is presented that does not compromise crystal quality, can reduce sample consumption by more than an order of magnitude and allows serial diffraction to be collected from a larger proportion of the crystals in the slurry.

摘要

高效的样品输送是同步加速器和X射线自由电子激光进行串行晶体学研究的一个重要方面。从样品消耗和束流时间利用的角度来看，使固定靶芯片在X射线下扫描是一种高效的串行输送方法。本文提出了一种利用声滴喷射加载固定靶的方法，该方法不会影响晶体质量，可将样品消耗降低一个数量级以上，并能从悬浮液中更大比例的晶体中收集串行衍射数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2af/6730619/6927fe2b50df/s-26-01820-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2af/6730619/61cc83309c1f/s-26-01820-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2af/6730619/7b1fe8abb3a7/s-26-01820-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2af/6730619/2a0135e3529c/s-26-01820-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2af/6730619/6927fe2b50df/s-26-01820-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2af/6730619/61cc83309c1f/s-26-01820-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2af/6730619/7b1fe8abb3a7/s-26-01820-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2af/6730619/2a0135e3529c/s-26-01820-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2af/6730619/6927fe2b50df/s-26-01820-fig4.jpg

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