Vanderbilt University Medical Center Vanderbilt-Ingram Cancer Center, Nashville, TN 37232-6838, USA.
Proc Am Thorac Soc. 2011 May;8(2):183-8. doi: 10.1513/pats.201012-069MS.
Since the advent of the new proteomics era more than a decade ago, large-scale studies of protein profiling have been exploited to identify the distinctive molecular signatures in a wide array of biological systems spanning areas of basic biological research, various disease states, and biomarker discovery directed toward therapeutic applications. Recent advances in protein separation and identification techniques have significantly improved proteomics approaches, leading to enhancement of the depth and breadth of proteome coverage. Proteomic signatures specific for invasive lung cancer and preinvasive lesions have begun to emerge. In this review we provide a critical assessment of the state of recent advances in proteomic approaches and the biological lessons they have yielded, with specific emphasis on the discovery of biomarker signatures for the early detection of lung cancer.
自十多年前新蛋白质组学时代的到来以来，人们已经利用大规模的蛋白质谱研究来鉴定广泛的生物系统中的独特分子特征，这些系统涵盖了基础生物学研究、各种疾病状态以及针对治疗应用的生物标志物发现等领域。蛋白质分离和鉴定技术的最新进展显著改进了蛋白质组学方法，从而提高了蛋白质组覆盖的深度和广度。针对侵袭性肺癌和癌前病变的蛋白质组学特征已经开始显现。在这篇综述中，我们批判性地评估了蛋白质组学方法的最新进展及其带来的生物学启示，特别强调了用于早期检测肺癌的生物标志物特征的发现。
