Scieux C, Bianchi A, Chappey B, Vassias I, Pérol Y
Laboratory of Bacteriology and Virology, Hôpital Saint-Louis, Université Paris VII, France.
J Antimicrob Chemother. 1990 Jan;25 Suppl A:7-10. doi: 10.1093/jac/25.suppl_a.7.
We evaluated the efficacy of azithromycin, erythromycin and doxycycline in controlling in-vitro propagation of Chlamydia trachomatis in HeLa 229 cells. Eight recent clinical isolates of C. trachomatis and two fast-growing strains were tested with inocula of 10(3)-10(5) inclusion forming units per well of a 96-well microtitre plate. C. trachomatis inclusions were detected by an immunoperoxidase-antiperoxidase procedure (PAP), including a genus-specific monoclonal antibody. MIC ranges were 0.064-0.25 mg/l azithromycin, 0.064-0.128 mg/l erythromycin and 0.016-0.064 mg/l doxycyline; MBC ranges were 2-8 mg/l azithromycin, 4-64 mg/l erythromycin and 0.5-8 mg/l doxycycline. Since azithromycin appears to be effective against C. trachomatis, clinical studies in sexually transmitted diseases are indicated.
我们评估了阿奇霉素、红霉素和强力霉素在体外控制沙眼衣原体在HeLa 229细胞中繁殖的效果。使用96孔微量滴定板，每孔接种10(3)-10(5)个包涵体形成单位，对8株近期临床分离的沙眼衣原体和2株快速生长菌株进行了测试。通过免疫过氧化物酶-抗过氧化物酶程序（PAP）检测沙眼衣原体包涵体，其中包括一种属特异性单克隆抗体。阿奇霉素的MIC范围为0.064-0.25mg/l，红霉素为0.064-0.128mg/l，强力霉素为0.016-0.064mg/l；MBC范围为阿奇霉素2-8mg/l，红霉素4-64mg/l，强力霉素0.5-8mg/l。由于阿奇霉素似乎对沙眼衣原体有效，因此有必要开展性传播疾病的临床研究。
