College of Pharmacy, Yeungnam University, Gyeongsan, 712-749, Korea.
Arch Pharm Res. 2011 Apr;34(4):687-93. doi: 10.1007/s12272-011-0420-9. Epub 2011 May 5.
A possible role of metabolism by intestinal bacteria in arbutin-induced toxicity was investigated in mammalian cell cultures. Following an incubation of arbutin with intestinal bacteria, either Bifidobacterium longum HY81 or Bifidobacterium adolescentis, for 24 h, its aglycone hydroquinone could be produced and detected in the bacterial culture media. The bacterial growth was not affected up to 10 mM arbutin in the culture medium. When the toxicity of bacteria cultured medium with arbutin was tested in the HepG2 cell lines, the medium with arbutin was more toxic than either parent arbutin only or bacteria cultured medium without arbutin, indicating that metabolic activation might be required in arbutin-induced toxicity. In addition, bacteria cultured medium with arbutin could suppress LPS and ConA mitogenicity in splenocyte cultures prepared from normal mice. The results indicate that the present toxicity testing system might be applied for assessing the possible role of metabolism by intestinal bacteria in certain chemical-induced toxicity in mammalian cell cultures.
研究了肠道细菌代谢对熊果苷诱导毒性的可能作用，采用哺乳动物细胞培养法进行研究。将熊果苷与肠道细菌（长双歧杆菌 HY81 或青春双歧杆菌）孵育 24 小时后，可在细菌培养物中产生并检测到其糖苷配基对苯二酚。在细菌培养物中，细菌的生长不受高达 10 mM 熊果苷的影响。当用 HepG2 细胞系检测含熊果苷的细菌培养物的毒性时，与仅用熊果苷或不含熊果苷的细菌培养物相比，含熊果苷的培养基毒性更强，表明熊果苷诱导的毒性可能需要代谢激活。此外，含熊果苷的细菌培养物可以抑制来自正常小鼠的脾细胞培养物中 LPS 和 ConA 的有丝分裂原活性。结果表明，本毒性测试系统可用于评估肠道细菌代谢在某些化学物质诱导的哺乳动物细胞培养物毒性中的可能作用。
