Dularay B, Elson C J, Clements-Jewery S, Damais C, Lando D
Medical School, University of Bristol, England.
J Leukoc Biol. 1990 Feb;47(2):158-63. doi: 10.1002/jlb.47.2.158.
Recombinant human Interleukin-1 (rhIL-1) beta was found to enhance stimulus-induced granule exocytosis from human polymorphonuclear leukocytes (PMNs). PMNs were incubated with rhIL-1 beta and then stimulated with either heat-aggregated IgG (Hagg) or N-formyl-methionyl leucylphenylalanine (FMLP). The release of the azurophil enzyme myeloperoxidase (MPO) was measured. Low concentrations of stimuli (10 micrograms/ml Hagg, 2.5 X 10(-9) M FMLP) did not stimulate degranulation in the absence of rhIL-1 beta. However, such concentrations elicited marked degranulation from PMNs preincubated with rhIL-1 beta (0.2-100 ng/ml). The enhancement of degranulation was dependent on the concentration of rhIL-1 beta employed and on the period of incubation. In other experiments, the effect of rhIL-1 beta on the PMN oxidative response was determined. rhIL-1 beta did not directly stimulate the production of superoxide anions or enhance the oxidative response to Hagg or FMLP. It is suggested that in rheumatoid joints, IL-1 beta may potentiate PMN degranulation, but not their oxidative response.
已发现重组人白细胞介素-1（rhIL-1）β可增强人多形核白细胞（PMN）刺激诱导的颗粒胞吐作用。将PMN与rhIL-1β孵育，然后用热聚集IgG（Hagg）或N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）刺激。测量嗜天青酶髓过氧化物酶（MPO）的释放。在没有rhIL-1β的情况下，低浓度刺激物（10微克/毫升Hagg，2.5×10^(-9) M FMLP）不会刺激脱颗粒。然而，这些浓度可引起与rhIL-1β（0.2 - 100纳克/毫升）预孵育的PMN显著脱颗粒。脱颗粒的增强取决于所用rhIL-1β的浓度和孵育时间。在其他实验中，确定了rhIL-1β对PMN氧化反应的影响。rhIL-1β不会直接刺激超氧阴离子的产生，也不会增强对Hagg或FMLP的氧化反应。提示在类风湿关节中，IL-1β可能增强PMN脱颗粒，但不会增强其氧化反应。
