Dularay B, Elson C J, Dieppe P A
Department of Pathology, University of Bristol.
Autoimmunity. 1988;1(3):159-69. doi: 10.3109/08916938808997161.
Polymorphonuclear leukocytes (PMN) isolated from the synovial fluid (SF) of patients with rheumatoid arthritis (RA) exhibited an enhanced oxidative response to heat aggregated IgG (Hagg) and N-formyl-methionyl-leucyl-phenylalanine (FMLP) as compared with either autologous or normal blood PMN. Normal blood PMN pretreated with synovial fluid showed a significantly increased response to FMLP which was unaffected by prior dialysis of SF. The degree of enhancement produced varied between SF's and was dependent on the period for which cells were incubated and on the concentration of SF used. In contrast there was no enhancement of the oxidative response when Hagg was used as a stimulus. Indeed, SF produced a dose dependent inhibition of Hagg stimulated superoxide production. These observations suggest that SF's from patients with RA contain factors which both enhance and inhibit the oxidative response of PMN depending on the subsequent stimulus.
与自体或正常血液中的多形核白细胞（PMN）相比，从类风湿性关节炎（RA）患者滑液（SF）中分离出的PMN对热聚集IgG（Hagg）和N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）表现出增强的氧化反应。用滑液预处理的正常血液PMN对FMLP的反应显著增加，而滑液预先透析对此无影响。不同滑液产生的增强程度不同，这取决于细胞孵育的时间和所用滑液的浓度。相反，当使用Hagg作为刺激物时，氧化反应没有增强。实际上，滑液对Hagg刺激的超氧化物产生具有剂量依赖性抑制作用。这些观察结果表明，RA患者的滑液含有一些因子，这些因子根据后续刺激既增强又抑制PMN的氧化反应。
