鉴定和表征蓝猪耳花粉管吸引剂 TcCRP1。
Identification and characterization of TcCRP1, a pollen tube attractant from Torenia concolor.
机构信息
Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan.
出版信息
Ann Bot. 2011 Sep;108(4):739-47. doi: 10.1093/aob/mcr111. Epub 2011 May 5.
Abstract
BACKGROUND AND AIMS
During sexual reproduction in higher angiosperms, the pollen tubes are directed to the ovules in the pistil to deliver sperm cells. This pollen tube attraction is highly species specific, and a group of small secreted proteins, TfCRPs, are necessary for this process in Torenia fournieri.
METHODS
A candidate pollen tube attractant protein in Torenia concolor, a related species of T. fournieri, was isolated and the attractant abilities between them were compared.
KEY RESULTS
TcCRP1, an orthologous gene of TfCRP1 from T. concolor, is expressed predominantly in the synergid cell. The gene product attracted pollen tubes in a concentration-dependent manner, but attracted fewer pollen tubes from the other species.
CONCLUSIONS
The results indicated that this class of CRP proteins is a common pollen tube attractant in Torenia species. The sequence diversity of these proteins is important for species-specific pollen tube attraction.
摘要
背景和目的
在高等被子植物的有性生殖过程中，花粉管被引导至雌蕊中的胚珠以输送精子细胞。这种花粉管吸引具有高度的物种特异性，而一组小的分泌型蛋白 TfCRPs 对于 Torenia fournieri 中的这个过程是必需的。
方法
从与 Torenia fournieri 相关的物种 Torenia concolor 中分离出候选花粉管吸引蛋白，并比较它们之间的吸引能力。
主要结果
TcCRP1 是 T. concolor 中 TfCRP1 的同源基因，主要在助细胞中表达。该基因产物以浓度依赖的方式吸引花粉管，但对来自其他物种的花粉管的吸引力较弱。
结论
结果表明，这类 CRP 蛋白是 Torenia 属中常见的花粉管吸引物。这些蛋白质的序列多样性对于物种特异性花粉管吸引很重要。
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PLoS Biol. 2010 Jun 1;8(6):e1000388. doi: 10.1371/journal.pbio.1000388.
3
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J Exp Bot. 2010 Apr;61(7):1987-99. doi: 10.1093/jxb/erq021. Epub 2010 Feb 24.
4
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Dev Comp Immunol. 2010 Jun;34(6):659-68. doi: 10.1016/j.dci.2010.01.012. Epub 2010 Feb 2.
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