Department of Biology, Faculty of Arts and Sciences, Gaziantep University, Gaziantep, Turkey.
Fish Physiol Biochem. 2011 Dec;37(4):951-8. doi: 10.1007/s10695-011-9492-1. Epub 2011 May 6.
The effects of dimethoate on hematological, biochemical parameters, and behavior were investigated in Oncorhynchus mykiss exposed to sublethal concentrations of 0.0735, 0.3675, and 0.7350 mg/l for 5, 15, and 30 days. Significant decrease was determined in erythrocyte and leukocyte counts, hemoglobin, hematocrit, MCV, and MCH, which was pronounced after prolonged exposure indicating the appearance of microcytic hypochromic anemia. There were no prominent changes in thrombocyte and MCHC. The glucose concentration showed an ascending pattern that proved to be positively correlated with duration. The protein concentration declined in higher dimethoate concentrations following 15 and 30 days. Negative and significant correlation was detected between glucose and protein concentrations. The fish showed remarkable behavioral abnormality such as loss of balance, erratic swimming, and convulsion. Present findings revealed that dimethoate exerts its toxic action even in sublethal concentrations and hematological parameters and abnormal behavior may be sensitive indicators to evaluate pesticide intoxication.
本研究旨在探讨久效磷对虹鳟（Oncorhynchus mykiss）血液学、生化参数和行为的影响。虹鳟分别暴露于 0.0735、0.3675 和 0.7350 mg/L 的久效磷亚致死浓度下 5、15 和 30 天。结果表明，红细胞和白细胞计数、血红蛋白、血细胞比容、MCV 和 MCH 显著降低，长时间暴露后更为明显，表明出现了小细胞低色素性贫血。血小板和 MCHC 没有明显变化。血糖浓度呈上升趋势，与时间呈正相关。15 和 30 天后，较高浓度的久效磷会导致蛋白质浓度下降。血糖和蛋白质浓度之间存在显著的负相关关系。鱼表现出明显的行为异常，如失去平衡、不规则游泳和抽搐。本研究结果表明，久效磷即使在亚致死浓度下也会产生毒性作用，血液学参数和异常行为可能是评估农药中毒的敏感指标。
