National Metal and Materials Technology Center, 114 Thailand Science Park, Paholyothin Rd, Klong 1, Klong Luang, Pathumthani 12120, Thailand.
J Biomed Mater Res A. 2011 Aug;98(2):185-91. doi: 10.1002/jbm.a.33104. Epub 2011 May 4.
The dressing prepared from GTMAC modified chitin-PAA was introduced with the aim of facilitating wound healing, particularly those effectively absorbing exudates, maintaining a moist wound environment and controlling bacterial proliferation. Chitin was chemically modified with acrylic acid to encourage a moist wound healing environment. The highly water-absorbable resulting product (chitin-PAA) was further reacted with glycidyltrimethylammonium chloride (GTMAC) to impart antibacterial activities. The final product, chitin-PAA-GTMAC was characterized by the techniques of Fourier Transform Infrared (FTIR), solid state (15) N NMR, and elemental analysis. Their cytotoxicity and antibacterial activities against S. epidermidis and E. coli were evaluated which found increasing effects in those properties with increasing degree substitution of GTMAC. All materials also showed good blood-clotting ability. The collagen gel contraction assay was used to analyze the behavior of fibroblasts after contact with the gels. The extent of the gel contraction as well as the examination of the secreted interleukin-8 (IL-8) and transforming growth factor-β1 (TGF-β1) were investigated. The results showed that chitin-PAA modified with GTMAC could stimulate the production of IL-8, but TGF-β1. Fibroblasts presented normal spreading and formation of cellular processes in the collagen gels with all of the modifications. Furthermore, all modified gels except for the highest GTMAC content gel [chitin-PAA-GTMAC (1:20)] were found a greater extent in gel contraction than the unmodified chitin-PAA. It suggested the promoting effect of GTMAC on cell proliferation if the GTMAC content in the gel was not too high, that is, the mole ratio of glucosamine to GTMAC of the gel should not greater than 1:10.
GTMAC 改性壳聚糖-PAA 制备的敷料旨在促进伤口愈合，特别是那些能够有效吸收渗出物、保持伤口湿润环境和控制细菌增殖的敷料。壳聚糖与丙烯酸进行化学改性，以促进湿润的伤口愈合环境。得到的高吸水性产物（壳聚糖-PAA）与缩水甘油三甲基氯化铵（GTMAC）进一步反应，赋予其抗菌活性。最终产物壳聚糖-PAA-GTMAC 通过傅里叶变换红外（FTIR）、固态（15）N NMR 和元素分析进行了表征。评估了它们对表皮葡萄球菌和大肠杆菌的细胞毒性和抗菌活性，发现随着 GTMAC 取代度的增加，这些性质的效果呈递增趋势。所有材料还表现出良好的凝血能力。使用胶原凝胶收缩测定法分析接触凝胶后成纤维细胞的行为。研究了凝胶收缩程度以及分泌的白细胞介素-8（IL-8）和转化生长因子-β1（TGF-β1）的检查。结果表明，用 GTMAC 改性的壳聚糖-PAA 可以刺激 IL-8 的产生，但不能刺激 TGF-β1 的产生。成纤维细胞在所有改性的凝胶中呈现出正常的伸展和细胞突起的形成，而在未改性的壳聚糖-PAA 中则没有。此外，除了 GTMAC 含量最高的凝胶[壳聚糖-PAA-GTMAC（1:20）]之外，所有改性的凝胶都发现其凝胶收缩程度大于未改性的壳聚糖-PAA。这表明在凝胶中 GTMAC 含量不太高的情况下，GTMAC 对细胞增殖有促进作用，即凝胶中氨基葡萄糖与 GTMAC 的摩尔比不应大于 1:10。
