Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Prannok Rd., Bangkok, Thailand.
J Mater Sci Mater Med. 2011 Nov;22(11):2497-504. doi: 10.1007/s10856-011-4420-6. Epub 2011 Aug 19.
Acrylic grafted chitin (chitin-PAA) was modified with glycidyltrimethylammonium chloride (GTMAC) with the aim of promoting wound healing. The chitin-PAA-GTMAC gels with different GTMAC contents were compared with the original chitin-PAA gel and Intrasite gel for their efficacy in deep wound healing of Wistar rats. Four full-thickness wounds were made on the dorsal skin of rats and then each was treated with 4 materials; chitin-PAA, chitin-PAA-GTMAC(1:4), chitin-PAA-GTMAC(1:10) and Intrasite gel. During 18 days of treatment, the wounds were visually observed and calculated for wound size using image analysis program. Skin wound tissues of sacrificed rats were processed for routine histological observation and immunohistochemistry of proliferating cell nuclear antigen (PCNA). The wounds covered with the chitin derivatives either with or without GTMAC showed a significant reduction in wound size in day 9 in comparison with day 12 for those covered with Intrasite gel. The faster rate and the better pattern of epidermal development observed in histological study as well as the higher dermal cell proliferation (PCNA expression) also demonstrated the better efficiency in wound healing of the chitin derivatives than Intrasite. The earliest epidermal development of the wounds treated with chitin-PAA-GTMAC (1:4) among the tested materials suggested the most promising of this material for the treatment of full-thickness open wound.
用氯化 3-二甲氨基丙基（GTMAC）对接枝丙烯酰胺的壳聚糖（壳聚糖-PAA）进行改性，目的是促进伤口愈合。用不同 GTMAC 含量的壳聚糖-PAA-GTMAC 凝胶与原始壳聚糖-PAA 凝胶和 Intrasite 凝胶进行比较，以评估它们对 Wistar 大鼠深部伤口愈合的疗效。在大鼠背部皮肤制造四个全层伤口，然后分别用 4 种材料进行处理：壳聚糖-PAA、壳聚糖-PAA-GTMAC（1:4）、壳聚糖-PAA-GTMAC（1:10）和 Intrasite 凝胶。在 18 天的治疗过程中，对伤口进行肉眼观察，并使用图像分析程序计算伤口大小。对处死大鼠的皮肤伤口组织进行常规组织学观察和增殖细胞核抗原（PCNA）的免疫组织化学分析。与 Intrasite 凝胶相比，用壳聚糖衍生物（无论是否用 GTMAC）覆盖的伤口在第 9 天的伤口面积明显小于第 12 天。组织学研究中观察到更快的表皮发育速度和更好的模式，以及更高的真皮细胞增殖（PCNA 表达），这也表明壳聚糖衍生物在伤口愈合方面比 Intrasite 凝胶具有更好的效果。与测试材料中的其他材料相比，用壳聚糖-PAA-GTMAC（1:4）处理的伤口最早出现表皮发育，这表明该材料最有希望用于治疗全层开放性伤口。
