Vargas-Alfredo Nelson, Munar-Bestard Marta, Ramis Joana Maria, Monjo Marta
Cell Therapy and Tissue Engineering Group, Department of Fundamental Biology and Health Sciences, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands, Ctra. Valldemossa Km 7.5, 07122 Palma de Mallorca, Spain.
Health Research Institute of the Balearic Islands (IdISBa), Ctra. Valldemossa 79, University Hospital Son Espases, Edificio S, 07120 Palma de Mallorca, Spain.
Gels. 2022 Oct 5;8(10):630. doi: 10.3390/gels8100630.
Gelatin methacryloyl (GelMA) hydrogels have been widely used for different biomedical applications due to their tunable physical characteristics and appropriate biological properties. In addition, GelMA could be modified with the addition of functional groups providing inherent antibacterial capabilities. Here, GelMA-based hydrogels were developed through the combination of a GelMA unmodified and modified polymer with quaternary ammonium groups (GelMAQ). The GelMAQ was synthesized from GelMA with a low degree of substitution of methacrylamide groups (DSMA) and grafted with glycidyltrimethylammonium chloride in the free amine groups of the lysine moieties present in the original gelatin. GelMAs with high DSMA and GelMAQ were combined 50/50% or 25/75% (/), respectively, and compared to controls GelMA and GelMA with added chlorhexidine (CHX) at 0.2%. The different hydrogels were characterized using H-NMR spectroscopy and swelling behavior and tested in (1) to evaluate their antibacterial properties and (2) human gingival fibroblast to evaluate their cell biocompatibility and regenerative properties. GelMA/GelMAQ 25/75% showed good antibacterial properties but also excellent biocompatibility and regenerative properties toward human fibroblasts in the wound healing assay. Taken together, these results suggest that the modification of GelMA with quaternary groups could facilitate periodontal tissue regeneration, with good biocompatibility and added antibacterial properties.
甲基丙烯酰化明胶（GelMA）水凝胶因其可调节的物理特性和适当的生物学特性而被广泛应用于不同的生物医学领域。此外，通过添加具有固有抗菌能力的官能团，可以对GelMA进行改性。在此，通过将未改性的GelMA和带有季铵基团的改性聚合物（GelMAQ）相结合，制备了基于GelMA的水凝胶。GelMAQ由甲基丙烯酰胺基团取代度较低（DSMA）的GelMA合成，并在原始明胶中赖氨酸部分的游离胺基团上接枝缩水甘油基三甲基氯化铵。分别将高DSMA的GelMA和GelMAQ按50/50%或25/75%（/）的比例混合，并与对照GelMA以及添加了0.2%洗必泰（CHX）的GelMA进行比较。使用核磁共振氢谱（H-NMR）光谱和溶胀行为对不同的水凝胶进行表征，并在（1）中进行测试以评估其抗菌性能，在（2）人牙龈成纤维细胞中进行测试以评估其细胞生物相容性和再生性能。在伤口愈合试验中，25/75%的GelMA/GelMAQ显示出良好的抗菌性能，同时对人成纤维细胞具有优异的生物相容性和再生性能。综上所述，这些结果表明，用季铵基团对GelMA进行改性可以促进牙周组织再生，具有良好的生物相容性和额外的抗菌性能。
