Department of Surgery, Harvard Medical School, Beth Israel Deaconess Medical Center, 110 Francis Street, Boston, MA 02215, USA.
Biomaterials. 2011 Aug;32(23):5371-9. doi: 10.1016/j.biomaterials.2011.04.009. Epub 2011 May 6.
Artificial composites designed to mimic the structure and properties of native extracellular matrix may lead to acellular materials for soft tissue repair and replacement, which display mechanical strength, stiffness, and resilience resembling native tissue. We describe the fabrication of thin lamellae consisting of continuous collagen microfiber embedded at controlled orientations and densities in a recombinant elastin-like protein polymer matrix. Multilamellar stacking affords flexible, protein-based composite sheets whose properties are dependent upon both the elastomeric matrix and collagen content and organization. Sheets are produced with properties that range over 13-fold in elongation to break (23-314%), six-fold in Young's modulus (5.3-33.1 MPa), and more than two-fold in tensile strength (1.85-4.08 MPa), exceeding that of a number of native human tissues, including urinary bladder, pulmonary artery, and aorta. A sheet approximating the mechanical response of human abdominal wall fascia is investigated as a fascial substitute for ventral hernia repair. Protein-based composite patches prevent hernia recurrence in Wistar rats over an 8-week period with new tissue formation and sustained structural integrity.
设计用于模拟天然细胞外基质结构和特性的人工复合材料可能会导致无细胞材料用于软组织修复和替代，这些材料具有类似于天然组织的机械强度、硬度和弹性。我们描述了在重组弹性蛋白样聚合物基质中以受控取向和密度嵌入连续胶原微纤维的薄层的制造。多层堆叠提供了柔韧的基于蛋白质的复合片材，其性能取决于弹性基质和胶原含量和组织。所制备的片材的断裂伸长率（23-314%）变化范围超过 13 倍，杨氏模量（5.3-33.1 MPa）变化范围超过 6 倍，拉伸强度（1.85-4.08 MPa）变化范围超过两倍，超过了许多天然人体组织，包括膀胱、肺动脉和主动脉。作为腹疝修复的筋膜替代品，研究了一种类似于人类腹壁筋膜机械响应的基于蛋白质的复合补片。在 8 周的时间内，基于蛋白质的复合补片阻止了 Wistar 大鼠的疝复发，同时形成了新的组织并保持了结构完整性。
