Department of Endocrinology and Metabolism, Ajou University School of Medicine, Suwon City, Gyeonggi Province, South Korea.
Eur J Pharmacol. 2011 Aug 16;664(1-3):54-9. doi: 10.1016/j.ejphar.2011.04.047. Epub 2011 May 1.
Poncirin, flavanone glycoside, isolated from the fruit of Poncirus trifoliata, has anti-bacterial and anti-inflammatory activities. In this study, the effects of poncirin on the differentiation of mesenchymal stem cells were investigated. The C3H10T1/2 mesenchymal stem cells and primary bone marrow mesenchymal stem cells were studied. In the C3H10T1/2 cells, poncirin prevented adipocyte differentiation, as demonstrated by inhibition of cytoplasm lipid droplet accumulation and peroxisome proliferator-activating receptor-γ (PPAR-γ) and CCAAT-enhancer-binding protein-β (C/EBP-β) mRNA expression. By contrast, poncirin enhanced the expression of the key osteogenic transcription factors, runt-related transcription factor 2 (Runx2) and transcriptional coactivator with PDZ-binding motif (TAZ). Poncirin also enhanced expression of the osteogenic marker genes including alkaline phosphatase (ALP) and osteocalcin (OC). Poncirin increased mineral nodule formation in primary bone marrow mesenchymal stem cells. These results suggest that poncirin prevents adipogenesis and enhances osteoblast differentiation in mesenchymal stem cells.
补骨脂素是从枳实果实中分离得到的一种类黄酮糖苷，具有抗菌和抗炎活性。本研究探讨了补骨脂素对间充质干细胞分化的影响。本研究以 C3H10T1/2 间充质干细胞和原代骨髓间充质干细胞为研究对象。在 C3H10T1/2 细胞中，补骨脂素通过抑制细胞质脂滴积累以及过氧化物酶体增殖物激活受体-γ（PPAR-γ）和 CCAAT 增强子结合蛋白-β（C/EBP-β）mRNA 的表达，阻止脂肪细胞分化。相比之下，补骨脂素增强了关键成骨转录因子 runt 相关转录因子 2（Runx2）和 PDZ 结合基序转录共激活因子（TAZ）的表达。补骨脂素还增强了碱性磷酸酶（ALP）和骨钙素（OC）等成骨标志物基因的表达。补骨脂素增加了原代骨髓间充质干细胞中矿化结节的形成。这些结果表明，补骨脂素可防止间充质干细胞的脂肪生成，并增强成骨细胞的分化。
