Lu Yan, Xi Wanpeng, Ding Xiaobo, Fan Shengjie, Zhang Yu, Jiang Dong, Li Yiming, Huang Cheng, Zhou Zhiqin
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China.
Int J Mol Sci. 2013 Dec 5;14(12):23736-50. doi: 10.3390/ijms141223736.
Obesity is becoming one of the global epidemics of the 21st century. In this study, the effects of citrange (Citrus sinensis × Poncirus trifoliata) fruit extracts in high-fat (HF) diet-induced obesity mice were studied. Female C57BL/6 mice were fed respectively a chow diet (control), an HF diet, HF diet supplemented with 1% w/w citrange peel extract (CPE) or 1% w/w citrange flesh and seed extract (CFSE) for 8 weeks. Our results showed that both CPE and CFSE regulated the glucose metabolic disorders of obese mice. In CPE and CFSE-treated groups, the body weight gain, blood glucose, serum total cholesterol (TC) and low density lipoprotein cholesterol (LDL-c) levels were significantly (p<0.05) reduced relative to those in the HF group. To explore the mechanisms of action of CPE and CFSE on the metabolism of glucose and lipid, related genes' expressions in liver were assayed. In liver tissue, the expression level of peroxisome proliferator-activated receptor γ (PPARγ) and its target genes were down-regulated by CPE and CFSE supplementation as revealed by qPCR tests. In addition, both CPE and CFSE decreased the expression level of liver X receptor (LXR) α and β, which are involved in lipid and glucose metabolism. Taken together, these results suggest that CPE and CFSE administration could ameliorate obesity and related metabolic disorders in HF diet-induced obesity mice probably through the inhibition of PPARγ and LXRs gene expressions.
肥胖正成为21世纪的全球性流行病之一。在本研究中，研究了枳橙（Citrus sinensis × Poncirus trifoliata）果实提取物对高脂（HF）饮食诱导的肥胖小鼠的影响。将雌性C57BL/6小鼠分别喂食普通饮食（对照）、高脂饮食、添加1% w/w枳橙果皮提取物（CPE）或1% w/w枳橙果肉和种子提取物（CFSE）的高脂饮食，持续8周。我们的结果表明，CPE和CFSE均能调节肥胖小鼠的葡萄糖代谢紊乱。在CPE和CFSE处理组中，相对于HF组，体重增加、血糖、血清总胆固醇（TC）和低密度脂蛋白胆固醇（LDL-c）水平显著（p<0.05）降低。为了探究CPE和CFSE对葡萄糖和脂质代谢的作用机制，检测了肝脏中相关基因的表达。qPCR检测显示，在肝脏组织中，CPE和CFSE补充剂下调了过氧化物酶体增殖物激活受体γ（PPARγ）及其靶基因的表达水平。此外，CPE和CFSE均降低了参与脂质和葡萄糖代谢的肝脏X受体（LXR）α和β的表达水平。综上所述，这些结果表明，给予CPE和CFSE可能通过抑制PPARγ和LXRs基因表达来改善HF饮食诱导的肥胖小鼠的肥胖及相关代谢紊乱。
