Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, A-6020 Innsbruck, Austria.
China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2012 Mar;62(Pt 3):710-715. doi: 10.1099/ijs.0.023937-0. Epub 2011 May 6.
Two psychrophilic strains, Cr7-05(T) and Cr4-44(T), isolated from alpine glacier cryoconite, were characterized by using a polyphasic approach. Both strains were psychrophilic, showing good growth over a temperature range of 1-20 °C. The chemotaxonomic characteristics of these isolates included the presence of C(18:1)ω7c and summed feature 3 (C(16:1)ω7c and/or C(16:1)ω6c) as the major cellular fatty acids, Q-10 as the predominant ubiquinone and diphosphatidylglycerol, phosphatidylglycerol and unknown glycolipids as major polar lipids. The DNA G+C contents of strains Cr7-05(T) and Cr4-44(T) were 61.4 and 63.6 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two isolates belong to the genus Devosia. The 16S rRNA gene sequence similarity between the two strains was 98.6%, but DNA-DNA hybridization indicated 54% relatedness. Strains Cr7-05(T) and Cr4-44(T) exhibited 16S rRNA gene sequence similarity of 94.7-97.2 and 94.9-96.9%, respectively, to the type strains of recognized Devosia species. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strains Cr7-05(T) and Cr4-44(T) represent two novel species within the genus Devosia, for which the names Devosia psychrophila sp. nov. (type strain Cr7-05(T) =DSM 22950(T) =CGMCC 1.10210(T) =CIP 110130(T)) and Devosia glacialis sp. nov. (type strain Cr4-44(T) =CGMCC 1.10691(T) =LMG 26051(T)) are proposed. An emended description of the genus Devosia is also provided.
从高山冰川冰核中分离到的两株嗜冷菌 Cr7-05(T) 和 Cr4-44(T)，采用多相分类法进行了特征描述。这两株菌均为嗜冷菌，在 1-20°C 的温度范围内生长良好。这些分离株的化学分类特征包括存在 C(18:1)ω7c 和总和特征 3（C(16:1)ω7c 和/或 C(16:1)ω6c）作为主要的细胞脂肪酸，Q-10 作为主要的泛醌，二磷脂酰甘油、磷脂酰甘油和未知糖脂作为主要的极性脂。菌株 Cr7-05(T) 和 Cr4-44(T) 的 DNA G+C 含量分别为 61.4 和 63.6mol%。基于 16S rRNA 基因序列的系统发育分析表明，这两株菌属于德沃斯氏菌属。两株菌的 16S rRNA 基因序列相似性为 98.6%，但 DNA-DNA 杂交表明它们的相关性为 54%。菌株 Cr7-05(T) 和 Cr4-44(T) 与公认的德沃斯氏菌种的 16S rRNA 基因序列相似性分别为 94.7-97.2%和 94.9-96.9%。基于表型特征、系统发育分析和 DNA-DNA 相关性数据，菌株 Cr7-05(T) 和 Cr4-44(T) 代表德沃斯氏菌属中的两个新种，分别命名为德沃斯氏菌嗜冷亚种（模式菌株 Cr7-05(T) =DSM 22950(T) =CGMCC 1.10210(T) =CIP 110130(T)）和德沃斯氏菌冰川亚种（模式菌株 Cr4-44(T) =CGMCC 1.10691(T) =LMG 26051(T)）。还提供了德沃斯氏菌属的修订描述。
