Key Laboratory of Marine Ecosystem Dynamics, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, 310012, Zhejiang, People's Republic of China.
Ocean College, Zhejiang University, Hangzhou, 310012, Zhejiang, People's Republic of China.
Antonie Van Leeuwenhoek. 2024 Jan 27;117(1):29. doi: 10.1007/s10482-023-01924-y.
A gram-stain-negative, aerobic, rod-shaped bacterium strain CJK-A8-3 was isolated from a polyamine-enriched seawater sample collected from the Changjiang River estuary of China. The colonies were white and circular. Strain CJK-A8-3 grew optimally at 35 °C, pH 7.0 and 1.5% NaCl. Its polar lipids contained phosphatidylglycerol, phosphatidic acid, unidentified glycolipids, and a combination of phospholipids and glycolipids. The respiratory quinone was ubiquinone-10, and its main fatty acids were C, 11-methyl Cω7c and Summed Feature 8 (including Cω7c/Cω6c). The phylogenetic tree based on 16S rRNA genes placed strain CJK-A8-3 in a new linage within the genus Devosia. 16S rRNA gene sequence of strain CJK-A8-3 showed identities of 98.50% with Devosia beringensis S02, 98.15% with D. oryziradicis, and 98.01% with D. submarina JCM 18935. The genome size of strain CJK-A8-3 was 3.81 Mb with the DNA G + C content 63.9%, higher than those of the reference strains (60.4-63.8%). The genome contained genes functional in the metabolism of terrigenous aromatic compounds, alkylphosphonate and organic nitrogen, potentially beneficial for nutrient acquirement and environmental remediation. It also harbored genes functional in antibiotics resistance and balance of osmotic pressure, enhancing their adaptation to estuarine environments. Both genomic investigation and experimental verification showed that strain CJK-A8-3 could be versatile and efficient to use diverse organic nitrogen compounds as carbon and nitrogen sources. Based on phenotypic, chemotaxonomic, phylogenetic and genomic characteristics, strain CJK-A8-3 was identified as a novel Devosia species, named as Devosia aquimaris sp. nov. The type strain is CJK-A8-3 (= MCCC 1K06953 = KCTC 92162).
一株革兰氏阴性、需氧、杆状细菌 CJK-A8-3 菌株从中国长江口富多胺海水样本中分离得到。菌落呈白色圆形。该菌株在 35°C、pH7.0 和 1.5%NaCl 条件下生长最佳。其极性脂含有磷脂酰甘油、磷脂酸、未鉴定的糖脂和磷脂与糖脂的组合。呼吸醌为泛醌-10，主要脂肪酸为 C、11-甲基 Cω7c 和总和特征 8（包括 Cω7c/Cω6c）。基于 16S rRNA 基因的系统发育树将菌株 CJK-A8-3 置于 Devosia 属的一个新谱系中。菌株 CJK-A8-3 的 16S rRNA 基因序列与 Devosia beringensis S02 的相似度为 98.50%，与 D. oryziradicis 的相似度为 98.15%，与 D. submarina JCM 18935 的相似度为 98.01%。菌株 CJK-A8-3 的基因组大小为 3.81Mb，GC 含量为 63.9%，高于参考菌株（60.4-63.8%）。基因组包含与陆地芳香族化合物、烷基膦酸盐和有机氮代谢相关的基因，这可能有利于获取营养和环境修复。它还拥有与抗生素抗性和渗透压平衡相关的基因，增强了它们对河口环境的适应能力。基因组调查和实验验证均表明，菌株 CJK-A8-3 可以灵活有效地利用多种有机氮化合物作为碳源和氮源。基于表型、化学分类、系统发育和基因组特征，菌株 CJK-A8-3 被鉴定为一种新的 Devosia 物种，命名为 Devosia aquimaris sp. nov. 模式菌株为 CJK-A8-3（=MCCC 1K06953=KCTC 92162）。
