Devosia aquimaris sp. nov., isolated from seawater of the Changjiang River estuary of China.

A gram-stain-negative, aerobic, rod-shaped bacterium strain CJK-A8-3 was isolated from a polyamine-enriched seawater sample collected from the Changjiang River estuary of China. The colonies were white and circular. Strain CJK-A8-3 grew optimally at 35 °C, pH 7.0 and 1.5% NaCl. Its polar lipids contained phosphatidylglycerol, phosphatidic acid, unidentified glycolipids, and a combination of phospholipids and glycolipids. The respiratory quinone was ubiquinone-10, and its main fatty acids were C, 11-methyl Cω7c and Summed Feature 8 (including Cω7c/Cω6c). The phylogenetic tree based on 16S rRNA genes placed strain CJK-A8-3 in a new linage within the genus Devosia. 16S rRNA gene sequence of strain CJK-A8-3 showed identities of 98.50% with Devosia beringensis S02, 98.15% with D. oryziradicis, and 98.01% with D. submarina JCM 18935. The genome size of strain CJK-A8-3 was 3.81 Mb with the DNA G + C content 63.9%, higher than those of the reference strains (60.4-63.8%). The genome contained genes functional in the metabolism of terrigenous aromatic compounds, alkylphosphonate and organic nitrogen, potentially beneficial for nutrient acquirement and environmental remediation. It also harbored genes functional in antibiotics resistance and balance of osmotic pressure, enhancing their adaptation to estuarine environments. Both genomic investigation and experimental verification showed that strain CJK-A8-3 could be versatile and efficient to use diverse organic nitrogen compounds as carbon and nitrogen sources. Based on phenotypic, chemotaxonomic, phylogenetic and genomic characteristics, strain CJK-A8-3 was identified as a novel Devosia species, named as Devosia aquimaris sp. nov. The type strain is CJK-A8-3 (= MCCC 1K06953 = KCTC 92162).