Department of Built Environment, School of engineering, Aalto University, P.O. Box 15200, Aalto, FI-00076, Espoo, Finland.
Institute of Water Research, University of Granada, C/Ramón y Cajal, 4, 18071, Granada, Spain.
Sci Rep. 2018 Feb 2;8(1):2208. doi: 10.1038/s41598-018-20633-5.
Seven full-scale biological wastewater treatment systems located in the Polar Arctic Circle region in Finland were investigated to determine their Archaea, Bacteria and Fungi community structure, and their relationship with the operational conditions of the bioreactors by the means of quantitative PCR, massive parallel sequencing and multivariate redundancy analysis. The results showed dominance of Archaea and Bacteria members in the bioreactors. The activated sludge systems showed strong selection of Bacteria but not for Archaea and Fungi, as suggested by diversity analyses. Core OTUs in influent and bioreactors were classified as Methanobrevibacter, Methanosarcina, Terrestrial Group Thaumarchaeota and unclassified Euryarchaeota member for Archaea; Trichococcus, Leptotrichiaceae and Comamonadaceae family, and Methylorosula for Bacteria and Trichosporonaceae family for Fungi. All influents shared core OTUs in all domains, but in bioreactors this did not occur for Bacteria. Oligotype structure of core OTUs showed several ubiquitous Fungi oligotypes as dominant in sewage and bioreactors. Multivariate redundancy analyses showed that the majority of core OTUs were related to organic matter and nutrients removal. Also, there was evidence of competition among Archaea and Fungi core OTUs, while all Bacteria OTUs were positively correlated among them. The results obtained highlighted interesting features of extremely cold temperature bioreactors.
为了确定在芬兰极地北极圈内的七个全规模生物污水处理系统的古菌、细菌和真菌群落结构与其生物反应器操作条件之间的关系，采用定量 PCR、大规模平行测序和多元冗余分析的方法对其进行了调查。结果表明，生物反应器中古菌和细菌成员占主导地位。活性污泥系统对细菌有很强的选择性，但对古菌和真菌没有选择性，多样性分析也表明了这一点。进水和生物反应器中的核心 OTU 被分类为古菌中的甲烷杆菌、甲烷球菌、陆地组奇古菌和未分类的广古菌成员；细菌中的 Trichococcus、Leptotrichiaceae 和 Comamonadaceae 科以及 Methylorosula；真菌中的 Trichosporonaceae 科。所有进水在所有领域都共享核心 OTU，但在生物反应器中，细菌并非如此。核心 OTU 的寡型结构显示出一些普遍存在的真菌寡型在污水和生物反应器中占主导地位。多元冗余分析表明，大多数核心 OTU 与有机物和营养物质的去除有关。此外，古菌和真菌核心 OTU 之间存在竞争的证据，而所有细菌 OTU 之间存在正相关。所得结果突出了极寒温度生物反应器的有趣特征。
