Department Of Pharmacology And Toxicology, Box 980613, Medical College Of Virginia, Richmond, VA 23298, USA.
Restor Neurol Neurosci. 1997 Jan 1;11(1):1-12. doi: 10.3233/RNN-1997-111201.
Male rats received bilateral frontal cortex contusions and were injected with 100 mg/kg of EGb 761 or an equal volume of vehicle beginning 5 min after injury and then with 1 injection/day for 7 days. The rats were tested for spontaneous motor behavior on days 1, 5, 10, and 15 postinjury and then for 10 days of spatial navigation performance in the Morris Water Maze (MWM), beginning on the day 8 after the contusion. Brain tissue was removed for examination on the 18th day after injury. Contused rats given EGb 761 performed more like intact rats on measures of spontaneous motor activity while vehicle-treated counterparts remained more active than either shams or EGb 761-treated animals by the conclusion of testing. Contusion-only rats were worse than shams on spatial performance, while those given EGb 761 were less impaired. Histological analyses indicated that EGb 761 failed to prevent loss of tissue at the primary site of impact. However, the extract reduced retrograde degeneration of neurons, gliosis in the thalamus, and ex vacuo hydrocephalus. EGb 761 treatment also decreased the loss of ChAT-positive neurons in the dorsomedial caudate-putamen and in the nucleus basalis magnocellularis (NBM). The results of this study indicate that EGb 761 could be a possible treatment for traumatic brain injury.
雄性大鼠接受双侧额叶皮质挫伤，并在损伤后 5 分钟给予 100mg/kg 的 EGb761 或等量的载体，然后每天注射 1 次，持续 7 天。大鼠在损伤后第 1、5、10 和 15 天进行自发运动行为测试，然后在Morris 水迷宫（MWM）中进行 10 天的空间导航性能测试，从挫伤后的第 8 天开始。在损伤后第 18 天取出脑组织进行检查。给予 EGb761 的挫伤大鼠在自发运动活动的测量中表现得更像完整的大鼠，而载体处理的对照组在测试结束时仍然比假手术或 EGb761 处理的动物更活跃。挫伤大鼠的空间表现比假手术差，而给予 EGb761 的大鼠则受损程度较轻。组织学分析表明，EGb761 未能防止撞击的主要部位的组织丢失。然而，该提取物减少了丘脑的神经元逆行变性、胶质增生和空泡性脑积水。EGb761 治疗还减少了尾状核背内侧和基底神经节大细胞核（NBM）中 ChAT 阳性神经元的丢失。这项研究的结果表明，EGb761 可能是一种治疗创伤性脑损伤的潜在方法。
