Department of Chemistry and Biochemistry, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, California 90095, United States.
Bioconjug Chem. 2011 Jun 15;22(6):1006-11. doi: 10.1021/bc100593m. Epub 2011 May 16.
We present a robust scheme for preparation of semiconductor quantum dots (QDs) and cognate partners in a conjugation ready format. Our approach is based on bis-aryl hydrazone bond formation mediated by aromatic aldehyde and hydrazinonicotinate acetone hydrazone (HyNic) activated peptide coated quantum dots. We demonstrate controlled preparation of antibody--QD bioconjugates for specific targeting of endogenous epidermal growth factor receptors in breast cancer cells and for single QD tracking of transmembrane proteins via an extracellular epitope. The same approach was also used for optical mapping of RNA polymerases bound to combed genomic DNA in vitro.
我们提出了一种制备半导体量子点（QDs）及其共轭配体的稳健方案，以达到连接准备的格式。我们的方法基于双芳基腙键的形成，由芳香醛和烟酰基腙丙酮腙（HyNic）激活肽包被的量子点介导。我们证明了抗体-QD 生物偶联物的可控制备，用于特异性靶向乳腺癌细胞内源性表皮生长因子受体，并通过细胞外表位对跨膜蛋白进行单个 QD 跟踪。同样的方法也用于体外结合到梳理基因组 DNA 的 RNA 聚合酶的光学作图。
