Bio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama, Japan.
J Fluoresc. 2012 May;22(3):931-44. doi: 10.1007/s10895-011-1032-y. Epub 2012 Jan 8.
Cancer stands as a leading cause of mortality worldwide and diagnostics of cancer still faces drawbacks. Optical imaging of cancer would allow early diagnosis, evaluation of disease progression and therapy efficiency. To that aim, we have developed highly biocompatible PEG functionalized cadmium chalcogenide based three differently luminescent quantum dots (QDs) (CdS, CdSe and CdTe). Folate targeting scheme was utilized for targeting cancer cell line, MCF-7. We demonstrate the biocompatibility, specificity and efficiency of our nanotool in detection of cancer cells sparing normal cell lines with retained fluorescence of functionalized QDs as parental counterpart. This is the first time report of utilizing three differently fluorescent QDs and we have detailed about the internalization of these materials and time dependent saturation of targeting schemes. We present here the success of utilizing our biocompatible imaging tool for early diagnosis of cancer.
癌症是全球主要的死亡原因，癌症的诊断仍然存在一些缺陷。癌症的光学成像是实现早期诊断、评估疾病进展和治疗效果的重要手段。为此，我们开发了高度生物相容性的聚乙二醇（PEG）功能化的碲化镉基三种不同发光量子点（QDs）（CdS、CdSe 和 CdTe）。利用叶酸靶向方案来靶向癌细胞系 MCF-7。我们证明了我们的纳米工具在检测癌细胞方面的生物相容性、特异性和效率，同时保留了功能化 QDs 的荧光作为亲本对照，而对正常细胞系没有影响。这是首次利用三种不同荧光 QDs 的报告，我们详细描述了这些材料的内化和靶向方案的时间依赖性饱和。我们在这里展示了利用我们的生物相容性成像工具进行癌症早期诊断的成功。
