Department of Ophthalmology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, China.
Neurosci Lett. 2013 Feb 28;539:43-7. doi: 10.1016/j.neulet.2013.01.038. Epub 2013 Feb 4.
The retinal pigment epithelium (RPE) is a multifunctional, monolayer of cells located between the neural retina and the choroicapillaris. γ-Aminobutyric acid (GABA) is the most important inhibitory neurotransmitter in the retina and GABA receptors are known to be present in chick retina, sclera and cornea. There is a report of genes involved in GABA receptor signaling being expressed in human RPE, however, whether GABA receptors are present in chick RPE is unknown.
Real time PCR and western blot were used to determine the expression of GABA receptors (alpha1 GABAA, GABABR2, and rho1 GABAC receptors) in isolated chicken RPE. Immunofluorescence using antibodies against one of the GABA receptor sub-types was used to determine receptor localization.
Both real-time PCR and western blot demonstrated that alpha1 GABAA, GABABR2 and rho1 GABAC receptors were expressed in isolated chick RPE. Immunofluorescence further demonstrated that GABA receptors were localized to the cell membrane and plasma of RPE cells.
Alpha1 GABAA, GABABR2 and rho1 GABAC receptors were expressed in chick RPE. The purpose of the GABA receptors within the RPE remains to be explored.
视网膜色素上皮（RPE）是位于神经视网膜和脉络膜毛细血管之间的一层多功能单层细胞。γ-氨基丁酸（GABA）是视网膜中最重要的抑制性神经递质，已知 GABA 受体存在于鸡的视网膜、巩膜和角膜中。有报道称参与 GABA 受体信号转导的基因在人 RPE 中表达，但鸡的 RPE 是否存在 GABA 受体尚不清楚。
实时 PCR 和 Western blot 用于确定分离的鸡 RPE 中 GABA 受体（α1 GABAA、GABABR2 和 rho1 GABAC 受体）的表达。使用针对 GABA 受体亚基之一的抗体进行免疫荧光染色，以确定受体定位。
实时 PCR 和 Western blot 均表明 α1 GABAA、GABABR2 和 rho1 GABAC 受体在分离的鸡 RPE 中表达。免疫荧光进一步表明 GABA 受体定位于 RPE 细胞的细胞膜和质膜。
α1 GABAA、GABABR2 和 rho1 GABAC 受体在鸡的 RPE 中表达。RPE 中 GABA 受体的目的仍有待探索。
