CellASIC Corporation, Hayward, CA 94545, USA.
Biomed Microdevices. 2011 Aug;13(4):753-8. doi: 10.1007/s10544-011-9545-3.
The ability to culture cells in three dimensional extracellular matrix (3D ECM) has proven to be an important tool for laboratory biology. Here, we demonstrate a microfluidic perfusion array on a 96-well plate format capable of long term 3D ECM culture within biomimetic microchambers. The array consists of 32 independent flow units, each with a 4 μl open-top culture chamber, and 350 μl inlet and outlet wells. Perfusion is generated using gravity and surface tension forces, allowing the array to be operated without any external pumps. MCF-10A mammary epithelial cells cultured in Matrigel in the microfluidic array exhibit acinus morphology over 9 days consistent with previous literature. We further demonstrated the application of the microfluidic array for in vitro anti-cancer drug screening.
在三维细胞外基质（3D ECM）中培养细胞的能力已被证明是实验室生物学的重要工具。在这里，我们展示了一种基于 96 孔板格式的微流控灌注阵列，该阵列能够在仿生微腔室内进行长期的 3D ECM 培养。该阵列由 32 个独立的流单元组成，每个流单元都有一个 4 μl 的开放式培养腔室和 350 μl 的入口和出口井。灌注是通过重力和表面张力产生的，允许该阵列在没有任何外部泵的情况下运行。在微流控阵列中的 Matrigel 中培养的 MCF-10A 乳腺上皮细胞在 9 天内表现出与先前文献一致的腺泡形态。我们进一步证明了该微流控阵列在体外抗癌药物筛选中的应用。
