Institute of Near-field Optics and Nano Technology, School of Physics and Optoelectronic Technology, Dalian University of Technology, Dalian 116024, People's Republic of China.
Appl Biochem Biotechnol. 2011 Sep;165(2):485-96. doi: 10.1007/s12010-011-9267-2. Epub 2011 May 10.
By using three physical techniques (atomic force microscopy (AFM), laser tweezers, and Raman spectroscopy), many excellent works in single-cell/molecule research have been accomplished. In this review, we present a brief introduction to the principles of these three techniques, and their capabilities toward single-cell/molecule research are highlighted. Afterward, the advances in single-cell/molecule research that have been facilitated by these three techniques are described. Following this, their complementary assets for single-cell/molecule research are analyzed, and the necessity of integrating the functions of these three techniques into one instrument is proposed.
利用原子力显微镜（AFM）、激光镊子和拉曼光谱学这三种物理技术，已经完成了许多单细胞/分子研究方面的优秀工作。在这篇综述中，我们简要介绍了这三种技术的原理，并强调了它们在单细胞/分子研究中的应用。随后，描述了这三种技术在单细胞/分子研究中的应用进展。接下来，分析了这三种技术在单细胞/分子研究中互补的特点，并提出了将这三种技术的功能整合到一台仪器中的必要性。
