Li Mengqiu, Boardman Dan G, Ward Andrew, Huang Wei E
Kroto Research Institute, Sheffield University, Sheffield, UK.
Methods Mol Biol. 2014;1096:147-53. doi: 10.1007/978-1-62703-712-9_12.
Single-cell Raman spectroscopy is a noninvasive and label-free technology for biochemical analysis of bacterial cells. A single-cell Raman spectrum functions as a metabolic "fingerprint" of an individual cell, which enables differentiation of cell types, physiological states, nutrient condition, and variable phenotypes. Raman tweezers combines single-cell Raman spectroscopy with optical laser tweezers to allow the identification and isolation of single living cells according to their Raman spectra. After cell sorting subsequent culturing and genomic sequencing has the potential to reveal totally new groups of microbes. Stable isotope probing with Raman tweezers offers a culture-independent toolbox to study genetic functions and physiology of unculturable microorganisms in the ecosystem.
单细胞拉曼光谱是一种用于细菌细胞生化分析的非侵入性、无标记技术。单细胞拉曼光谱可作为单个细胞的代谢“指纹”，用于区分细胞类型、生理状态、营养状况和可变表型。拉曼镊子将单细胞拉曼光谱与光学激光镊子相结合，可根据单细胞的拉曼光谱对其进行识别和分离。细胞分选后进行后续培养和基因组测序，有可能揭示全新的微生物群体。利用拉曼镊子进行稳定同位素探测，为研究生态系统中不可培养微生物的遗传功能和生理学提供了一个不依赖培养的工具包。
