Department of Transfusion, The First Hospital of China Medical University, North Nanjing Street, No. 155, 110001 Shenyang, Liaoning, People's Republic of China.
Med Oncol. 2012 Jun;29(2):1397-402. doi: 10.1007/s12032-011-9972-2. Epub 2011 May 10.
The aim of this study is to evaluate the performance of VEGF and endostatin levels in the differential diagnosis of malignant and benign ascites. The study included 101 consecutive patients with malignant ascites (55.2 ± 15.8 years, 63 men and 38 women) and 81 patients with benign ascites (53.0 ± 17.2 years, 51 men and 30 women). VEGF and endostatin levels in serum and ascites were determined by a sandwich enzyme immunoassay technique using a commercially available assay kit. The serum VEGF, ascites VEGF, and ascites endostatin levels of patients with malignant ascites were significantly higher than those in patients with benign ascites (P < 0.001), but there was no difference in serum endostatin levels between the two groups (P = 0.267). Ascites endostatin levels correlated positively with ascites VEGF (r = 0.5193, P < 0.01), and serum endostatin showed a low correlation with serum VEGF (r = 0.3291, P < 0.01) in patients with malignant ascites. Areas under the ROC curves of ascites VEGF, ascites endostatin, serum VEGF, and serum endostatin were 0.890, 0.815, 0.694, and 0.552, respectively. The combination of ascites VEGF and endostatin improved the sensitivity up to 90.1%, the specificity up to 87.7%, and the accuracy up to 89.0% in the differential diagnosis of malignant and benign ascites. VEGF and endostatin levels in ascites appear to be suitable for differentiating between malignant and benign ascites, which can be applied to clinical examination.
本研究旨在评估 VEGF 和内皮抑素水平在良恶性腹水鉴别诊断中的作用。研究纳入 101 例恶性腹水患者（55.2±15.8 岁，男 63 例，女 38 例）和 81 例良性腹水患者（53.0±17.2 岁，男 51 例，女 30 例）。采用商用夹心酶联免疫吸附试验技术检测血清和腹水 VEGF 和内皮抑素水平。恶性腹水患者的血清 VEGF、腹水 VEGF 和腹水内皮抑素水平明显高于良性腹水患者（P<0.001），但两组血清内皮抑素水平无差异（P=0.267）。腹水内皮抑素水平与腹水 VEGF 呈正相关（r=0.5193，P<0.01），恶性腹水患者血清内皮抑素与血清 VEGF 呈低度相关（r=0.3291，P<0.01）。腹水 VEGF、腹水内皮抑素、血清 VEGF 和血清内皮抑素的 ROC 曲线下面积分别为 0.890、0.815、0.694 和 0.552。腹水 VEGF 和内皮抑素联合应用可将恶性和良性腹水的鉴别诊断敏感性提高至 90.1%，特异性提高至 87.7%，准确性提高至 89.0%。腹水 VEGF 和内皮抑素水平似乎适合于良恶性腹水的鉴别诊断，可应用于临床检查。
