Laboratorio de Medicina Regenerativa, Hospital Carlos Haya, Fundación IMABIS, Pabellón de Gobierno, Málaga, Spain.
Br J Pharmacol. 2011 Dec;164(7):1899-915. doi: 10.1111/j.1476-5381.2011.01477.x.
The lack of safe and effective treatments for obesity has increased interest in natural products that may serve as alternative therapies. From this perspective, we have analysed the effects of daidzein, one of the main soy isoflavones, on diet-induced obesity in rats.
Rats made obese after exposure to a very (60%) high fat-content diet were treated with daidzein (50 mg·kg(-1)) for 14 days. The dose was selected on the basis of the acute effects of this isoflavone on a feeding test. After 14 days, animals were killed and plasma, white and brown adipose tissue, muscle and liver studied for the levels and expression of metabolites, proteins and genes relevant to lipid metabolism.
A single treatment (acute) with daidzein dose-dependently reduced food intake. Chronic treatment (daily for 14 days) reduced weight gain and fat content in liver, accompanied by high leptin and low adiponectin levels in plasma. While skeletal muscle was weakly affected by treatment, both adipose tissue and liver displayed marked changes after treatment with daidzein, affecting transcription factors and lipogenic enzymes, particularly stearoyl coenzyme A desaturase 1, a pivotal enzyme in obesity. Expression of uncoupling protein 1, an important enzyme for thermogenesis, was increased in brown adipose tissue after daidzein treatment.
These results support the use of isoflavones in diet-induced obesity, especially when hepatic steatosis is present and open a new field of use for these natural products.
由于缺乏安全有效的肥胖症治疗方法，人们对可能作为替代疗法的天然产物产生了浓厚的兴趣。从这个角度出发，我们分析了大豆异黄酮主要成分之一大豆苷元对大鼠饮食诱导肥胖的影响。
让暴露于高（60%）脂肪含量饮食中的大鼠肥胖，然后用大豆苷元（50mg·kg(-1)）治疗 14 天。该剂量是基于该异黄酮在摄食试验中的急性作用选择的。14 天后，处死动物，研究血浆、白色和棕色脂肪组织、肌肉和肝脏中与脂质代谢相关的代谢物、蛋白质和基因的水平和表达。
单次（急性）大豆苷元处理剂量依赖性地降低了食物摄入量。慢性治疗（每天 14 天）减少了体重增加和肝脏中的脂肪含量，同时血浆中的瘦素水平升高，脂联素水平降低。虽然骨骼肌受治疗影响较弱，但脂肪组织和肝脏在大豆苷元治疗后均显示出明显变化，影响转录因子和脂肪生成酶，特别是在肥胖中起关键作用的硬脂酰辅酶 A 去饱和酶 1。解偶联蛋白 1 的表达，棕色脂肪组织产热的重要酶，在大豆苷元治疗后增加。
这些结果支持在饮食诱导肥胖症中使用异黄酮，特别是在存在肝脂肪变性时，并为这些天然产物开辟了新的应用领域。
