Department of Biology, University of Rochester, NY 14627, USA.
Integr Comp Biol. 2010 Nov;50(5):768-77. doi: 10.1093/icb/icq121. Epub 2010 Aug 30.
The snail Ilyanassa obsoleta is a useful model for a variety of investigations in the fields of developmental biology, cell biology, larval ecology, ecotoxicology, parasitology, and chemical ecology. To enhance such studies, we have carried out two cDNA sequencing projects to characterize the mRNA transcripts that are present during development of this embryo. These efforts have generated 480 megabases of new sequence, which have been assembled into transcript contigs and represent thousands of newly identified Ilyanassa genes. We identified the orthologs of 182 transcription factors in these data, focusing on families that are likely to be sequence-specific transcriptional regulators. To demonstrate the utility of identifying and examining such transcripts, we describe the expression pattern during organogenesis for IoOnecut, an Ilyanassa ortholog of the HNF6/onecut family of transcription factors.
美洲鲍螺（Ilyanassa obsoleta）是发育生物学、细胞生物学、幼虫生态学、生态毒理学、寄生虫学和化学生态学等领域各种研究的有用模式生物。为了促进这些研究，我们进行了两个 cDNA 测序项目，以描述在胚胎发育过程中存在的 mRNA 转录本。这些努力产生了 4.8 亿碱基对的新序列，这些序列已被组装成转录本，代表了数千个新鉴定的美洲鲍螺基因。我们在这些数据中鉴定了 182 个转录因子的直系同源物，重点关注可能是序列特异性转录调节因子的家族。为了证明鉴定和检查这些转录本的效用，我们描述了在器官发生过程中，HNF6/onecut 家族转录因子的美洲鲍螺同源物 IoOnecut 的表达模式。
