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细胞周期蛋白依赖性激酶（CDKs）是调控泥东风螺早期发育的关键基因：来自比较转录组和蛋白质组分析的证据

Cyclin-dependent kinases (CDKs) are key genes regulating early development of Neptunea arthritica cumingii: evidence from comparative transcriptome and proteome analyses.

作者信息

Lv Fengxiao, Ge Xinfan, Chang Yaqing, Hao Zhenlin

机构信息

Key Laboratory of Mariculture and Stock Enhancement in North China's Sea (Dalian Ocean University), Ministry of Agriculture, Dalian, 116023, China.

College of Fisheries and Life Science, Dalian Ocean University, 52, Heishijiao Street, Shahekou District, Dalian, Liaoning Province, China.

出版信息

BMC Genomics. 2024 Dec 19;25(1):1221. doi: 10.1186/s12864-024-10970-3.

DOI:10.1186/s12864-024-10970-3
PMID:39701993
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11660575/
Abstract

In this study, we applied comparative transcriptomics and proteomics techniques to systematically investigate the dynamic expression patterns of genes and proteins at various stages of early embryonic development of the gastropod Neptunea arthritica cumingii. Twelve cyclin-dependent kinase (CDKs) genes and five downstream proteins associated with these CDKs were identified. Through techniques such as qRT-PCR, our data elucidate for the first time the regulatory functions of CDK family genes and establish CDKs as a pivotal gene cluster in the early embryonic development of N. cumingii. These findings not only enhance the understanding of molecular developmental biology in N. cumingii and marine gastropods in general but also provide significant insights into the mechanisms involved in early embryonic development in N. cumingii. Furthermore, our results provide theoretical guidance for advancing artificial breeding technology for N. cumingii.

摘要

在本研究中，我们应用比较转录组学和蛋白质组学技术，系统地研究了腹足纲生物泥东风螺早期胚胎发育各阶段基因和蛋白质的动态表达模式。鉴定出了12个细胞周期蛋白依赖性激酶（CDK）基因以及与这些CDK相关的5种下游蛋白质。通过qRT-PCR等技术，我们的数据首次阐明了CDK家族基因的调控功能，并确立了CDK作为泥东风螺早期胚胎发育中的关键基因簇。这些发现不仅增进了对泥东风螺及一般海洋腹足纲生物分子发育生物学的理解，还为泥东风螺早期胚胎发育所涉及的机制提供了重要见解。此外，我们的结果为推进泥东风螺人工养殖技术提供了理论指导。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddc9/11660575/ef72cc7c9111/12864_2024_10970_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddc9/11660575/357299601950/12864_2024_10970_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddc9/11660575/4f2b57200800/12864_2024_10970_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddc9/11660575/cfe171389323/12864_2024_10970_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddc9/11660575/ef72cc7c9111/12864_2024_10970_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddc9/11660575/357299601950/12864_2024_10970_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddc9/11660575/94768f68ec11/12864_2024_10970_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddc9/11660575/b7669dc139b3/12864_2024_10970_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddc9/11660575/5d08cdeddbd1/12864_2024_10970_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddc9/11660575/4f2b57200800/12864_2024_10970_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddc9/11660575/cfe171389323/12864_2024_10970_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddc9/11660575/ef72cc7c9111/12864_2024_10970_Fig7_HTML.jpg

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Cyclin E/CDK2: DNA Replication, Replication Stress and Genomic Instability.细胞周期蛋白E/细胞周期蛋白依赖性激酶2：DNA复制、复制应激与基因组不稳定
Front Cell Dev Biol. 2021 Nov 24;9:774845. doi: 10.3389/fcell.2021.774845. eCollection 2021.
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