Harnett G B, Farr T J, Pietroboni G R, Bucens M R
Virus Laboratory, Queen Elizabeth II Medical Centre, Nedlands, Western Australia.
J Med Virol. 1990 Feb;30(2):128-30. doi: 10.1002/jmv.1890300209.
We have previously reported the isolation of HHV-6 from saliva samples. Because these isolations were made in PHA-stimulated lymphocytes from healthy adults, which may occasionally contain endogenous HHV-6, it was desirable to repeat this work using cord blood lymphocytes. In this study 18 isolations of viruses provisionally characterized as HHV-6 were made from 19 saliva samples by centrifugally enhanced inoculation into PHA-stimulated cord blood lymphocytes. HHV-6 was not found in 10 pernasal aspirates, 50 endocervical swabs, or 30 male urethral swabs. It is concluded that HHV-6 is usually present in the saliva of most adults and that this affords a possible explanation of the high infection rate with this virus in young children.
我们之前曾报道过从唾液样本中分离出HHV-6。由于这些分离是在来自健康成年人的PHA刺激的淋巴细胞中进行的，而这些淋巴细胞可能偶尔含有内源性HHV-6，因此有必要使用脐血淋巴细胞重复这项工作。在本研究中，通过离心增强接种到PHA刺激的脐血淋巴细胞中，从19份唾液样本中进行了18次初步鉴定为HHV-6的病毒分离。在10份鼻吸液、50份宫颈拭子或30份男性尿道拭子中未发现HHV-6。结论是HHV-6通常存在于大多数成年人的唾液中，这为该病毒在幼儿中的高感染率提供了一种可能的解释。
