Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei, Taiwan, ROC.
J Sci Food Agric. 2011 Sep;91(12):2284-91. doi: 10.1002/jsfa.4456. Epub 2011 May 10.
Immunomodulation by probiotic microorganisms has become a topic of increasing interest in food microbiology. Polysaccharides are broadly used in the food industry as gelling, thickening, stabilizing, or emulsifying agents. Some probiotics such as lactic acid bacteria also produce exopolysaccharides that stimulate macrophage production of cytokines. The aim of this study was to characterize the effects of exopolysaccharides of Lactobacillus paracasei subsp. paracasei NTU 101 (101EP) and Lactobacillus plantarum NTU 102 (102EP) exopolysaccharides on antioxidant activity and immunomodulation in vitro.
The sugar composition (including arabinose, galactose, glucose, fructose, mannose, and maltose) of 101EP and 102EP was quantified by high-performance anion-exchange chromatography. Cytokine production (including IL-6, TNF-α, and IL-1β) was induced by 101EP and 102EP in Raw 264.7 in a dose-dependent manner (5-500 µg mL(-1) ). 101EP and 102EP also demonstrated potential antioxidant properties (1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, chelation of ferrous ions, inhibition of linoleic acid peroxidation, and reducing power) in vitro.
101EP and 102EP stimulate cell proliferation and may be useful as a mild immune modulator of macrophages.
益生菌微生物的免疫调节作用已成为食品微生物学中日益关注的话题。多糖广泛应用于食品工业中，作为凝胶、增稠、稳定或乳化剂。一些益生菌，如乳酸菌，也会产生能刺激巨噬细胞产生细胞因子的胞外多糖。本研究旨在探讨副干酪乳杆菌亚种副干酪乳杆菌 NTU 101（101EP）和植物乳杆菌 NTU 102（102EP）胞外多糖对体外抗氧化活性和免疫调节的影响。
采用高效阴离子交换色谱法对 101EP 和 102EP 的糖组成（包括阿拉伯糖、半乳糖、葡萄糖、果糖、甘露糖和麦芽糖）进行了定量分析。101EP 和 102EP 以剂量依赖的方式（5-500 µg mL(-1)）诱导 Raw 264.7 细胞产生细胞因子（IL-6、TNF-α 和 IL-1β）。101EP 和 102EP 还表现出体外潜在的抗氧化特性（1,1-二苯基-2-苦基肼自由基清除活性、亚铁离子螯合能力、抑制亚油酸过氧化和还原能力）。
101EP 和 102EP 刺激细胞增殖，可能作为巨噬细胞的温和免疫调节剂有用。
