CSIC-Centro de Edafología y Biología Aplicada del Segura, Department of Soil and Water Conservation, P.O. Box 164, Campus de Espinardo 30100-Murcia, Spain.
Sci Total Environ. 2011 Jul 1;409(15):2874-80. doi: 10.1016/j.scitotenv.2011.04.029. Epub 2011 May 10.
The positive effect of arbuscular mycorrhizal fungi inoculation on plant establishment under field conditions has been shown. However, whether this effect is related to the survival of the AMF and how the AMF inoculum affects the colonisation of plant roots by the native AMF remain uncertain. In this study, we assessed the AMF community composition in O. europaea roots inoculated "in situ" with three types of AMF inoculum: Glomus sp., G. intraradices and a mixture of Glomus sp. and G. intraradices. All the AMF isolates inoculated showed a good percentage of persistence. Mycorrhizal inoculation with the mixture of Glomus sp. and G. intraradices was the most effective treatment for increasing the AMF diversity in roots 14 months after plantation. Plant growth was increased significantly by all the inocula tested, although plant biomass was not correlated with variation in the AMF diversity or with the AMF inoculum persistence. Thus, it seems that this positive effect was mediated by the interaction between the AMF inoculum and the natural colonisation. Therefore, the application of native AMF isolates in the planting hole may be considered a good strategy for the revegetation of semiarid, degraded soils, in order to reactivate the indigenous AMF populations and improve the performance of O. europaea seedlings, particularly when mixtures of native isolates are used.
已证明丛枝菌根真菌（AMF）接种对田间条件下植物定植的积极影响。然而，这种影响是否与 AMF 的存活有关，以及 AMF 接种物如何影响植物根系对本地 AMF 的定植仍然不确定。在这项研究中，我们评估了接种“原位”三种 AMF 接种物（Glomus sp.、G. intraradices 和 Glomus sp.和 G. intraradices 的混合物）的欧洲山杨（O. europaea）根系中的 AMF 群落组成。所有接种的 AMF 分离物都表现出良好的存活率。在定植后 14 个月，用 Glomus sp.和 G. intraradices 的混合物进行菌根接种是增加根系中 AMF 多样性的最有效处理方法。所有测试的接种物都显著增加了植物的生长，尽管植物生物量与 AMF 多样性的变化或 AMF 接种物的存活无关。因此，这种积极影响似乎是由 AMF 接种物与自然定植之间的相互作用介导的。因此，在种植孔中应用本地 AMF 分离物可能被认为是半干旱退化土壤植被恢复的一种良好策略，以便重新激活本地 AMF 种群并提高欧洲山杨幼苗的性能，特别是当使用本地分离物的混合物时。
