Department of Medicine, Weill Cornell Medical College, New York, NY 10065, USA.
Trends Microbiol. 2011 Jul;19(7):307-14. doi: 10.1016/j.tim.2011.03.008. Epub 2011 May 10.
Recent advances in liquid chromatography and mass spectrometry have enabled the highly parallel, quantitative measurement of metabolites within a cell and the ability to trace their biochemical fates. In Mycobacterium tuberculosis (Mtb), these advances have highlighted major gaps in our understanding of central carbon metabolism (CCM) that have prompted fresh interpretations of the composition and structure of its metabolic pathways and the phenotypes of Mtb strains in which CCM genes have been deleted. High-throughput screens have demonstrated that small chemical compounds can selectively inhibit some enzymes of Mtb's CCM while sparing homologs in the host. Mtb's CCM has thus emerged as a frontier for both fundamental and translational research.
近年来，液相色谱和质谱技术的发展使得在细胞内进行代谢物的高通量、定量测量以及追踪其生化命运成为可能。在结核分枝杆菌（Mtb）中，这些进展凸显了我们对中心碳代谢（CCM）理解的主要空白，促使人们对其代谢途径的组成和结构以及 CCM 基因缺失的 Mtb 菌株的表型进行了新的解释。高通量筛选表明，小分子化合物可以选择性地抑制 Mtb 的 CCM 中的一些酶，而宿主中的同源物则不受影响。因此，Mtb 的 CCM 成为基础和转化研究的前沿。
