Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, PR China.
Tree Physiol. 2011 May;31(5):567-73. doi: 10.1093/treephys/tpr032. Epub 2011 May 10.
Expression of the two coleopteran-resistant proteins (Bt-Cry3A and oryzacystatin I) was detected in the leaves of field-grown transgenic poplar (BOGA-5) in two or three subsequent years. The BOGA-5 contained ∼10 μg g(-1) of Cry3A over the individual years with no detection in the control, and protein extracts from BOGA-5 displayed a higher reduction in papain activity (∼42%) compared with ∼21% in the control. Laboratory feeding experiments showed that the total mortality of the target pest Plagiodera versicolora (Coleoptera, Chrysomelida) larvae fed with BOGA-5 leaves was 76.7%, significantly higher than that of the control (P< .05). However, no significant differences were detected in the mortality, exuviation index, pupation rate or adult eclosion rate of the non-target Clostera anachoreta (Lepidoptera, Notodontidae) fed with leaves from transgenic and non-transgenic poplars. Field investigation indicated that the transgenic poplar retained coleopteran insect resistance in the field, suggesting the potential use of the double gene transgenic poplar for pest management in commercial poplar plantations.
在随后的两三年里，在田间生长的转 Bt 杨树（BOGA-5）叶片中检测到了两种抗鞘翅目蛋白（Cry3A 和 oryzacystatin I）的表达。BOGA-5 个体年份中 Cry3A 的含量约为 10 μg g(-1)，而对照中未检测到，并且 BOGA-5 的蛋白提取物与对照相比，木瓜蛋白酶活性的降低幅度更高（约 42%），而对照为约 21%。实验室饲养实验表明，以 BOGA-5 叶片为食的靶标害虫黄杨绢野螟（鞘翅目，叶甲科）幼虫的总死亡率为 76.7%，显著高于对照（P< .05）。然而，以转基因此及非转基因杨树叶片为食的非靶标丽纹夜蛾（鳞翅目，枯叶蛾科）幼虫的死亡率、蜕皮指数、化蛹率或成虫羽化率均无显著差异。田间调查表明，转基因杨树在田间保持了对鞘翅目昆虫的抗性，这表明双基因转基因杨树有可能用于商业杨树人工林的害虫管理。
