Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland.
Analyst. 2011 Jun 21;136(12):2635-42. doi: 10.1039/c1an00005e. Epub 2011 May 12.
A new approach to the rapid determination of amoxicillin (AMO) in human plasma followed by solid phase microextraction (SPME) fiber coatings based on conducting polymers (polypyrrole and polythiophene) and high performance liquid chromatography (HPLC) has been described. The porous structures of the electrochemically deposited polymer coatings have been characterized by scanning electron microscopy (SEM). The experimental parameters relating to the extraction efficiency of the SPME fibers such as pH, extraction time and desorption conditions (solvents, time) were studied and selected. The SPME/HPLC-UV method was linear over a working range of 1-50 μg ml(-1). The inter-day accuracy (expressed as coefficients of variations, CVs) was less than 15% and precision (expressed as the relative standard deviations, RSDs) with percentage values was less than 5.9%. Amoxicillin was found to be stable in the human plasma at room temperature (20 °C) within 8 hours. The developed method was successfully applied to the analysis of real human plasma samples. The limit of detection and limit of quantification for amoxicillin in plasma were 1.21 μg ml(-1) and 3.48 μg ml(-1), respectively.
一种新的方法来快速测定阿莫西林（AMO）在人血浆中固相微萃取（SPME）纤维涂层基于导电聚合物（聚吡咯和聚噻吩）和高效液相色谱（HPLC）。电化学沉积聚合物涂层的多孔结构进行了扫描电子显微镜（SEM）的特点。萃取效率的实验参数有关的 SPME 纤维如 pH 值，萃取时间和洗脱条件（溶剂，时间）进行了研究和选择。SPME / HPLC - UV 法在工作范围内线性 1-50μgml（-1）。日内精度（表示为变异系数，CVs）小于 15%，精度（表示为相对标准偏差，RSDs）的百分比值小于 5.9%。阿莫西林被发现在室温下（20°C）在人血浆中 8 小时内稳定。所建立的方法被成功地应用于分析真实的人血浆样品。检测限和定量限的阿莫西林在血浆中分别为 1.21μgml（-1）和 3.48μgml（-1）。
