Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada.
Proteomics. 2011 May;11(10):2027-37. doi: 10.1002/pmic.201000631.
The anaerobic, Gram-negative bacillus Fusobacterium nucleatum plays a vital role in oral biofilm formation and the development of periodontal disease. The organism plays a central bridging role between early and late colonizers within dental plaque and plays a protective role against reactive oxygen species. Using a two-dimensional gel electrophoresis and mass spectrometry approach, we have annotated 78 proteins within the proteome of F. nucleatum subsp. nucleatum and identified those proteins whose apparent intracellular concentrations change in response to either O(2)- or H(2)O(2)-induced oxidative stress. Three major protein systems were altered in response to oxidative stress: (i) proteins of the alkyl hydroperoxide reductase/thioredoxin reductase system were increased in intracellular concentration; (ii) glycolytic enzymes were modified by oxidation (i.e. D-glyceraldehyde 3-phosphate dehydrogenase, and fructose 6-phosphate aldolase) or increased in intracellular concentration, with an accompanying decrease in ATP production; and (iii) the intracellular concentrations of molecular chaperone proteins and related proteins (i.e. ClpB, DnaK, HtpG, and HrcA) were increased.
产黑色素无芽胞杆菌核梭杆菌是一种厌氧的革兰氏阴性杆菌，在口腔生物膜形成和牙周病的发展中起着至关重要的作用。该生物在牙菌斑的早期和晚期定植者之间起着中心桥接作用，并对活性氧起到保护作用。使用二维凝胶电泳和质谱分析法，我们在产黑色素无芽胞杆菌亚种核梭杆菌的蛋白质组中注释了 78 种蛋白质，并鉴定了那些在对 O(2)-或 H(2)O(2)-诱导的氧化应激的反应中，细胞内浓度发生变化的蛋白质。有三个主要的蛋白质系统对氧化应激有反应：（i）烷基氢过氧化物还原酶/硫氧还蛋白还原酶系统的蛋白质细胞内浓度增加；（ii）糖酵解酶被氧化修饰（即 D-甘油醛 3-磷酸脱氢酶和果糖 6-磷酸醛缩酶）或细胞内浓度增加，同时伴随着 ATP 生成减少；（iii）分子伴侣蛋白和相关蛋白（即 ClpB、DnaK、HtpG 和 HrcA）的细胞内浓度增加。
