Shikimi T, Suzuki S, Takahashi M, Kaneto H
Department of Pharmacology, Shimane Medical University, Japan.
Scand J Clin Lab Invest. 1990 Feb;50(1):1-8. doi: 10.3109/00365519009091558.
In the enzyme-immunoassay, using rabbit anti-urinastatin (anti-UT) Fab'-beta-D-galactosidase complex and rabbit anti-UT IgG-coupled polystyrene ball as a solid phase, the least measurable amount of UT was 0.3 ng per assay tube. The assay was used to detect UT-like immunoreactivity in the urine of mice. The urine contained a UT-like immunoreactive substance with trypsin inhibitory activity, the concentration of which was 2.3 +/- 0.3 micrograms/l (mean +/- SEM, n = 10). Excretion in the urine was affected by glucocorticoids. There was no immunoreactivity against rabbit anti-UT IgG in the urine of rats, guinea-pigs, rabbits, cats and dogs.
在酶免疫测定中，以兔抗尿抑胃素（抗UT）Fab'-β-D-半乳糖苷酶复合物和兔抗UT IgG偶联的聚苯乙烯球作为固相，每个测定管中尿抑胃素的最低可测量量为0.3纳克。该测定法用于检测小鼠尿液中的尿抑胃素样免疫反应性。尿液中含有一种具有胰蛋白酶抑制活性的尿抑胃素样免疫反应性物质，其浓度为2.3±0.3微克/升（平均值±标准误，n = 10）。尿液排泄受糖皮质激素影响。大鼠、豚鼠、兔子、猫和狗的尿液中未检测到针对兔抗UT IgG的免疫反应性。
