Shikimi T, Suzuki S, Wessel T, Joh T H, Hattori K, Takaori S
Department of Pharmacology, Shimane Medical University, Japan.
Life Sci. 1992;50(19):1399-406. doi: 10.1016/0024-3205(92)90258-q.
Mouse liver contains a human urinary trypsin inhibitor (urinastatin, UT)-like immunoreactive substance with trypsin inhibitory activity. Northern blot analysis demonstrates the presence of the appropriate 1.3 kb mRNA band in liver tissue but not in kidney or other tissues examined. Administration of hydrocortisone, which is known to increase the urinary excretion of the UT-like substance, increased the levels of UT-like substance in serum and in the liver tissue. In contrast, deoxycorticosterone acetate did not have such an effect. These results suggest that the gene encoding UT-like substance is primarily expressed in the liver of the mouse, and that glucocorticoids play an important role in regulating the hepatic synthesis of UT-like substance. Furthermore, these findings indicate that the mouse is a suitable species for research on the biological function of UT or UT-like substances.
小鼠肝脏含有一种具有胰蛋白酶抑制活性的人尿胰蛋白酶抑制剂（乌司他丁，UT）样免疫反应性物质。Northern印迹分析表明，在肝脏组织中存在适当的1.3 kb mRNA条带，而在肾脏或其他检测的组织中则不存在。已知氢化可的松可增加UT样物质的尿排泄，给予氢化可的松可提高血清和肝脏组织中UT样物质的水平。相比之下，醋酸脱氧皮质酮没有这种作用。这些结果表明，编码UT样物质的基因主要在小鼠肝脏中表达，并且糖皮质激素在调节肝脏中UT样物质的合成中起重要作用。此外，这些发现表明小鼠是研究UT或UT样物质生物学功能的合适物种。
